聚L-半胱氨酸/DNA-HRP混合自组装膜修饰的过氧化氢传感器的研究  

A Novel Hydrogen Peroxide Biosensor Based on HRP-DNA/Poly-(L-Cys) Modified Pt Electrode

作　　者：谢轶[1] 袁若[1] 柴雅琴[1] 童忠强[1] 唐明宇[1] 

机构地区：[1]西南大学化学化工学院分析科学研究所,重庆400715

出　　处：《西南大学学报（自然科学版）》2007年第5期44-48,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(29705001);教育部优秀青年教师资助计划项目(200240);重庆市自然科学基金资助项目(20027477)

摘　　要：采用循环伏安法在铂电极表面聚合L半胱氨酸制得修饰电极,再利用DNA与蛋白质的特异性结合,将小牛胸腺DNA与辣根过氧化物酶依次滴加到聚L-Cys/Pt电极表面,制备得到DNA-HRP/L-Cys/Pt电极,实验证实该电极对过氧化氢具有响应快、灵敏度高、线性范围宽、稳定性好的性能,且具有良好的选择性.线性范围为1·2×10-6～9·0×10-3mol·L-1,检出限:8·0×10-7mol·L-1.A novel hydrogen peroxide（H2O2） biosensor based on DNA-HRP and Poly-L-cysteine modified Pt electrode was presented in this paper. Firstly, L-cysteine was polymerized on the bare Pt electrode by cyclic voltammetry （CV）.Then DNA-HRP was immobilized on the L-Cys/Pt surface by the method of layer by layer. The electrode offers an excellent electrochemical response for determination of H2O2 concentration below micromole level with high sensitivity and selectivity. The linear range for H2O2 determination was from 1.2 ×10^-6—9.0×10^-3 tool·L^-1 with a detection limit of 8.0×10^-7 mol·L^-1（S/N=3）.

关 键 词：聚合物修饰电极 L-半胱氨酸 过氧化物酶 循环伏安法 

分 类 号：O657[理学—分析化学]