正己烷静式染毒致SD大鼠生殖腺损伤  

Experimental research in sex glands of SD rats after n-hexane inhalation

作　　者：曹静婷[1] 黄中新[1] 程欣[1] 陈瑛[2] 

机构地区：[1]暨南大学医学院,广州510632 [2]广州市第十二人民医院

出　　处：《中华劳动卫生职业病杂志》2007年第5期275-278,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广东省医学科研基金(A2006566);广州市医药卫生科技项目(2005-YB-102)

摘　　要：目的观察正己烷静式吸人染毒对SD大鼠卵巢和睾丸所致的病理变化,并探讨正己烷对生殖腺的毒性作用及其机制。方法48只成年SD大鼠,雌雄各半,随机分为3个染毒组(1、3和7d)和1个对照组,采用SD大鼠正己烷静式吸入染毒法进行染毒。染毒结束处死动物后,测全血正己烷浓度,并取睾丸或卵巢称量计算脏器系数。取材后的生殖腺,一侧制组织匀浆测定谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量;另一侧用多聚甲醛固定做常规石蜡切片观察病理学变化。结果随染毒时间延长,染毒组的睾丸或卵巢组织中SOD、GSH、GSH-Px活力均降低,而MDA含量升高,尤以7d组最为明显,差异有统计学意义(P<0.01),显示正己烷染毒所致的不同程度的脂质过氧化损伤。镜下观察7d组生殖腺及发育中生殖细胞的微细结构出现明显异常。结论正己烷吸入染毒对大鼠生殖腺及发育中的生殖细胞有肯定的损伤作用,脂质过氧化损伤可能是毒性作用的机制之一。Objective To observe the pathological changes in ovary or testis of the SD rats after n-hexane inhalation, and to investigate the effects of n-hexane to sexual glands. Methods Forty-eight adult healthy rats were selected and randomizedly divided into four groups： control group and three n-hexane groups （1, 3 and 7 d）, six rats for each group. After inhalation, SD rats were killed and n-hexane concentration of blood h-om celiac arterywere detected. Super-oxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, malondialdehyde （MDA）, glutathione （GSH） were measured by photometry, and organ coefficients were calculated. The ovary or testis were embedded with paraffin, sliced, stained by HE method and observed using microscope. Results As the time of n-Hexane effect increased, the activities of SOD, GSH-Px and GSH were decreased, but the content of MDA was increased, especial in the 7 d group （P〈0.01）. Conclusions n-bexane can induce a series of damages in ovary or testis of SD rats, and lipid-preoxidation may be one of the effect process.

关 键 词：己烷类 生殖 脂质过氧化作用 

分 类 号：R114[医药卫生—卫生毒理学]