多色引物原位标记技术快速检测精子染色体  

Rapid Chromosome Detection by Multicolor PRINS in Human Sperm

作　　者：刘涤石[1] 丁显平[1] 魏萍[1] 王欢[1] 

机构地区：[1]生物资源与生态环境教育部重点实验室,四川大学生命科学学院遗传医学研究所,成都610064

出　　处：《生殖与避孕》2007年第5期338-342,共5页Reproduction and Contraception

摘　　要：目的:探索和评估应用于精子染色体数目异常检测的多色引物原位标记技术。方法:采用NaOH溶液对精子核进行去浓缩和变性,以非ddNTP阻断的单色及三色引物原位标记技术对正常男性精液标本的4条染色体进行检测。结果:成功地在2.5h内标记了同一精子核内的多条染色体,最佳的染色体同时标记通量为3条,单色以上标记达到99%。结论:该快速多色引物原位标记技术优化了之前的单色精子标记技术,在提高了检测通量的同时,检测也更为简便快速和经济可靠。Objective： To explore and evaluate a multicolor PRINS protocol for chromosome detection m human sperm. Methods： Two autosomes and 2 gonosomes in semen samples from 2 normal fertile men were analyzed using the non-ddNTP-blocking multicolor PRINS procedure and efficient NaOH decondensation and denaturation pretreatment. Results： Within 2.5 h, 3 chromosomes had been simultaneously marked in same sperm nucleus. The best detection flux was up to triple color for suitable application of sperm multi-PRINS reaction, and the frequency of one-color-labeling reached 99%. Conclusion： By optimizing the single-color PRINS protocol before, this multicolor protocol should enable fast, simple, sensitive and reliable chromosome identification, even increase the detection flux.

关 键 词：多色引物原位标记 精子 染色体 非整倍性 二倍体 

分 类 号：Q343-3[生物学—遗传学]