不同剂量乙醇对大鼠心肌糖代谢信号传递分子mRNA表达的影响  

Ethanol decreases the mRNA expression of the molecules involved in glucose metabolism in hearts of rats

作　　者：侯晓磊[1] 陶迎[1] 冯丽[1] 王来成[1] 马春燕[1] 完强[1] 高聆[1] 赵家军[1] 

机构地区：[1]山东大学山东省立医院科研中心

出　　处：《山东大学学报（医学版）》2007年第4期360-364,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2001C12)

摘　　要：目的:观察长期摄入不同剂量乙醇对大鼠心肌糖代谢传导通路中主要信号IR、IRS1、IRS2、GLUT4、AMPKα、MEF2基因转录水平的影响,探讨乙醇影响葡萄糖转运的可能机制。方法:雄性Wistar大鼠48只,随机分为对照组、大、中、小剂量乙醇组。乙醇喂养22周,留取左心室肌于液氮中保存。采用RT-PCR检测心肌IR、IRS1、IRS2、GLUT4、AMPKα1和AMPKα2亚基、MEF2A和MEF2D亚型mRNA的表达。结果:与正常对照组相比,除低剂量乙醇组MEF2D mRNA水平无明显变化外,其它饮酒组中各观察指标表达均下降(P<0.01或P<0.05)。除IR外,各饮酒组IRS1、IRS2、GLUT4、AMPKα、MEF2A、MEF2D mRNA水平的下降程度与摄入乙醇量存在剂量依赖关系,即摄入乙醇量越大,下降越明显。相关分析表明,GLUT4 mRNA的改变与IRS1、IRS2、AMPKα、MEF2A、MEF2D的变化呈正相关,其中GLUT4的表达水平与IRS1、MEF2A有较高相关性(r=0.683,r=0.824,P<0.01)。结论:长期摄入乙醇可降低大鼠心肌糖代谢信号分子的表达。Objective： To observe the effects of chronic ethanol consumption at different doses on mRNA levels of IR, IRS1, IRS2, GLUT4, AMPK and MEF2 and to explore the possible mechanisms of ethanol on the glucose transportation. Methods： Forty-eight male Wistar rots, randomly divided into four groups, received either distilled water （control group） or ethanol of three different dosages： 5 g/（ kg· d） （ high dose group）, 2.5 g/（ kg· d） （ middle dose group）, and 0.5 g/（kg·d） （low dose group）. After treatment for 22 weeks, all animals were sacrificed and the left ventricles were quickly removed and saved for later analysis. The mRNA levels of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D were determined by the RT-PCR method. Results： Compared with the control group, all mRNA levels of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D were decreased in ethanol treatment groups （especially in the high dose and middle dose groups） （P 〈 0.05）. Correlation analysis indicated that GLUT4 had a significantly positive correlation with IRS1, IRS2, AMPKα1, AMPKα2, MEF2A or MEF2D. Of them, it was closely associated between GLUT4 and IRS1 or MEF2A （ P 〈 0.01 ）. Conclusions： Chronic ethanol consumption impairs the mRNA expression of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D in the cardiac muscle in rats.

关 键 词：乙醇 葡萄糖转运子4 蛋白激酶 AMP激活 肌细胞增强因子 大鼠 Wistar 

分 类 号：R58[医药卫生—内分泌]