神经移位重建高位颈髓损伤大鼠呼吸功能后神经的组织化学特点研究  

The histochemical study on transpositional nerve for respiratory function rehabilitation in rats model with upper cervical spinal cord injuries

作　　者：周许辉[1] 叶晓健[1] 袁文[1] 贾连顺[1] 严望军[1] 

机构地区：[1]第二军医大学附属长征医院骨科,上海200003

出　　处：《脊柱外科杂志》2007年第2期106-108,共3页Journal of Spinal Surgery

基　　金：国家自然科学基金资助项目(30571886)

摘　　要：目的从吻合神经的组织化学变化特点来探讨副神经移位膈神经重建高位颈髓损伤大鼠呼吸功能的可行性。方法健康雄性SD大鼠60只。随机分为1、2、3、4、5、6个月6个时间组。取下颈部正中切口,将双侧副神经在锁骨下水平发出内、外侧支之前切断,与膈神经干起始部移位缝接。术后第1、2、3、4、5、6个月各组均取颈部正中切口显露两侧吻合的神经。分别于吻合口远、近端5 mm处取材。用亚铁氰化铜法进行乙酰胆碱酯酶(AChE)组织化学染色。利用VIDS-Ⅲ型图像分析仪进行运动纤维计数,远端与近端相除后得出远端运动纤维通过率。结果神经吻合后随着时间的延长,吻合口远端运动纤维通过率逐渐增加。第1个月的通过率为43.06%±5.36%。第6个月为97.55%±6.72%。结论副神经移位膈神经后,神经运动纤维能较理想地通过吻合口向远端生长,为恢复膈肌的神经支配模式,重建高位颈髓损伤大鼠呼吸功能创造了有利条件。Objective To assess the possibility of anastomosad nerve for respiratory rehabilitation in rats model with upper cervical spinal cord injuries （ucSCI）. Methods Sixty male adult rats underwent transposition of accessory nerve and phrenic nerve. Accessory nerve was cut subolavicularly upside the embranchment of the interior and exterior branches, and was sutured with phrenic nerve trunk on both sides. The nerve fthers in 5ram distal an proximal to the anastomosis were get monthly after operation, in ten rats each time. Ache histoehemically stain was performed with Kamorsky-roos methods. The motor fibers were analyzed by the VIDS- Ⅲ graphical machine. The passing rate of the motor fibers in the distal anastomosis was ealeulatod. Results The passing rate of motor fthers was 43. 06 ±5. 36% atl mon, and97.55±6.72% at6 mons. Conclusion The motor fibers could pass anastomosis after transposition of accessory nerve and phrenie nerve in rats model with ueSCI. It shows the potential possibility to rehabilitate respiratory ftmetion by nerve transporsition.

关 键 词：膈神经 副神经 脊髓损伤 组织细胞化学 

分 类 号：R651.21[医药卫生—外科学]