应用自动化工作站提取常见生物样本DNA  

Study on the application of the automation workstation in DNA extraction of biological samples

作　　者：严江伟[1] 王顺霞[1] 任贺[2] 荆玉婷[3] 高林林[4] 唐晖[1] 焦章平[1] 刘雅诚[1] 

机构地区：[1]北京市公安局法医检验鉴定中心 [2]北京市人民警察学院,北京100088 [3]山西医科大学,山西太原030001 [4]杭州市公安局,浙江杭州310004

出　　处：《中国法医学杂志》2007年第2期73-75,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立使用自动化工作站提取法医案件生物样本DNA的方法。方法选用Biomek 3000自动化工作站,采用DNA IQTM系统及Chelex法对法医案件中常见生物样本进行DNA提取,荧光定量技术进行定量,PCR扩增16个STR基因座并与手工提取方法比较。结果与手工提取方法进行比较,选用自动化工作站结合使用DNAIQTM系统及Chelex法提取DNA可获得满意的STR检验结果。结论自动化工作站可用于法医案件中常见生物样本的DNA提取。Objective To study the application of the automation workstation in DNA extraction of forensic casework samples. Methods The Biomek 3000 laboratory automation workstation with the DNA IQTM System and Chelex-100 DNA extraction program was applied to DNA extraction of forensic casework samples. All DNA samples were quantitated by the fluorescent quantitation technique and genotyped subsequently using the Powerplex 16 system. These results were compared with those from manual DNA extraction methods. Results The DNA amount and success ratio of STR analysis with the automation workstation were closed to those from manual DNA extraction methods. Conclusion The Biomek 3000 Laboratory Automation Workstation can be applied in DNA extraction of forensic casework samples.

关 键 词：法医物证学 自动化工作站 DNA提取 定量 STR检验 

分 类 号：D919[医药卫生—法医学]