用于固定抗体的新型温敏性核-壳结构聚合物微球的合成及其初步应用研究  

SYNTHESIS OF A NOVEL TYPE OF THERMOSENSITIVE CORE-SHELL POLYMER MICROSPHERES FOR IMMOBILIZATION OF ANTIBODY AND THEIR PRIMARY APPLICATION

作　　者：孟庆斌[1] 李湛勇[1] 李刚[1] 张栩[1] 朱晓夏[1] 

机构地区：[1]教育部功能高分子材料重点实验室南开大学高分子化学研究所,天津300071

出　　处：《离子交换与吸附》2007年第3期193-198,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金杰出青年B基金资助(No.20328407)

摘　　要：利用无皂乳液聚合和种子聚合的方法合成了一种以聚苯乙烯为核,聚(N-异丙基丙烯酰胺-co-N-丙烯酸琥珀酰亚胺酯)为壳的单分散的核-壳结构的聚合物微球。用扫描电镜和透射电镜观察了球的形貌特征,发现微球具有清晰的核-壳结构和较好的单分散性,红外光谱显示了在1738cm-1处有酯羰基的特征吸收峰。动态光散射测定发现该聚合物微球具有温敏性,当温度高于聚N-异丙基丙烯酰胺的最低临界溶液温度(LCST)时,球的流体力学直径变小。利用微球壳层所含有的琥珀酰亚胺酯基与伯氨基的高反应活性,将抗体Rabbit IgG化学固定在球的壳层上。由于壳层的聚N-异丙基丙烯酰胺具有温敏性,反应温度不同结合的抗体的量也不同,在0℃和36.5℃,微球对抗体的结合率分别为61.6%和38.6%。The cow-shell microspheres were synthesized by surfactant-free emulsion polymerization and seed polymerization with polystyrene as the core and poly（N-isopropylacry!amide-co-N-acryloxysuccinimide） as the shell. The core-shell morphology of the microspheres was studied by scanning electron microscopy and transmission electron microscopy, indicating clear core-shell structure and well monodisperse morphology. The composition of the microspheres was determined by FT-IR spectroscopy, which indicated the successful preparation because of the appearance of the peak at 1738cm^-1. The thermosensitiveness of the microspheres was detected by dynamic light scattering, and when the temperature was increased over the lower critical solution temperature （LCST） of the poly（N-isopropylacrylamide）, the hydrodynamic diameter decreased. The antibody was chemically conjugated onto the microspheres through the reaction between primary amino groups and the succinimide ester groups due to their high reactivity. The conjugation ratios of the antibody are 61.6% and 38.6% at 0℃ and 36.5℃, respectively, due to their thermosensitiveness.

关 键 词：无皂乳液聚合 核-壳结构 温敏性的 抗体固定 

分 类 号：TQ425[化学工程]