CD4^+CD25^+T细胞对NK细胞活性的影响  

Effect of CD4^+CD25^+T cells on activation of natural killer cells

作　　者：王庆红[1] 王晓娟[1] 甘成军[1] 彭旭[1] 罗高兴[1] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆市疾病蛋白质组学重点实验室

出　　处：《重庆医学》2007年第11期1055-1057,共3页Chongqing medicine

基　　金：重庆市自然基金项目(STC;2006BB5071)

摘　　要：目的初步探讨CD4+CD25+T细胞对NK细胞杀伤活性的影响,以及TGF-β1分子在其中的作用。方法磁珠分选纯化BALB/c小鼠脾脏CD4+CD25+T细胞与NK细胞,体外用ConA激活CD4+CD25+T细胞,将激活的CD4+CD25+T细胞与NK细胞共培养,并加入抗TGF-β1抗体,在共培养的24h和48h,用51Cr标记的YAC-1检测NK细胞的杀伤毒性。结果初始CD4+CD25+T细胞在与NK细胞共培养的24h和48h能够显著抑制NK细胞活性,其抑制率分别为50.8%和49.1%,加入抗TGF-β1抗体后,抑制率变为:-0.1%、0.25%;ConA激活的CD4+CD25+T细胞在与NK细胞共培养的24h和48h也能显著抑制NK细胞活性,其抑制率分别为19%和20.9%,加入抗TGF-β1抗体后,抑制率变为25.2%、16.3%。结论TGF-β1参与了CD4+CD25+T细胞对NK细胞毒性的抑制,并且,其在CD4+CD25+T细胞激活、不激活状态下的影响结果不同。Objective To explore the possible role of CD4^＋CD25^＋T cells stimulated with ConA on natural killer（NK） cells and to determine the effect of TGF-β1 molecule on this actions. Methods CD4^＋CD25^＋ T cells and NK cells were obtained from Balb/e mice splenoeytes by MACS. Fresh isolated CD4^＋CD25^＋ T cells activated by ConA in vitro for 24h then auto-NK cells were added into the culture. Anti-TGF-β1 antibody was added into the co-culture system. ^51Cr labeled YAC-1 was used to detect NK cells eototoeixity at 24h and 48h. Results Naive CD4^＋CD25^＋ T displayed a suppressive activity on NK cells and the inhibition rate was 50.8% at 24h and 49.1% at 48h. The inhibition rate of ConA activated CD4^＋CD25^＋ T cells was 19% at 24h and 20.9% at 48h. When anti-TGF-β1 antibody added, the inhibition rate of naive T cells was -0. 1% and 0.25% was respectively while that of ConA activated T cells was 25.2%and 16.3% kespeetively. Conclusion In vitro, CD4^＋CD25^＋ T cells stimulated by Con A can more significantly inhibit the function of NK cells than naive CD4^＋CD25^＋ T cells. TGF-β1 plays different roles on this action in different inhibition systems.

关 键 词：CD4^+CD25^+T细胞 NK细胞 抗-TGF-β1抗体 细胞毒性 

分 类 号：R392[医药卫生—免疫学]