大豆花叶病毒(SMV)侵染大豆种胚的研究  

ON THE SOYBEAN EMBRYO INVASION BY SOYBEAN MOSAIC VIRUS

作　　者：董宏平[1,2] 濮祖茂[1,2] 濮祖芹[1,2] 

机构地区：[1]南京农业大学植物保护学系 [2]中国药科大学

出　　处：《南京农业大学学报》1997年第1期35-38,共4页Journal of Nanjing Agricultural University

摘　　要：采用大豆病、健株正反交和病、健株自交的方法，以间接ＥＬＩＳＡ检测其后代种胚的带毒率。试验结果表明：♀（Ｓ）×（Ｓ）、♀（Ｓ）×（Ｈ）两组合子代的种胚带毒率较高，在结荚初期均超过６０％；♀（Ｈ）×（Ｓ）子代种胚带毒率较低，仅２５％左右；在健株自交组合中未检测到病毒。说明胚珠在发育早期受到病毒的侵染可能是种子带毒的主要因素，而带毒的花粉使种胚受到病毒侵染的作用较小。对大豆花前期病、健株子房组织进行免疫胶体金扫描及超薄切片观察的结果也证实。Indirect ELISA was used to detect the percentage of infected embryos of F 1 hybride in four crosses. The result showed that: the incidence of SMV infected embryos from ♀(S)×(S) and ♀(S)×(H) crosses was higher than that of the others. It was exceeded 60% in the beginning of podding, whereas it was only about 25% from the ♀(H)×(S) cross. It was suggested that the principal role of soybean embryo infection was by invasion of the ovules. By scanning electron microscope (SEM), immunogold labelling was widely located in the ovary wall, on the surface of the ovules and cleaving planes of them in SMV infected plants. The bandles of SMV like particles were also found in the ovary walls and embryos sac in the thin sections of soybean ovaries.

关 键 词：大豆花叶病毒 大豆 胚 胚珠 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]