反相高效液相色谱法测定微乳凝胶剂中鬼臼毒素的含量  

RP-HPLC determination of podophyllotoxin in microemulsion-based gel

作　　者：杜丹荣[1] 陈华兵[2] 常雪灵[2] 牟东升[1] 朱丹丹[2] 郑静南[2] 杨祥良[2] 

机构地区：[1]华中科技大学化学系,武汉430074 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《中国新药杂志》2007年第10期788-791,共4页Chinese Journal of New Drugs

基　　金：湖北省科技攻关项目(2005AA301B10)

摘　　要：目的:建立测定鬼臼毒素微乳凝胶剂中鬼臼毒素含量的方法。方法:采用反相高效液相色谱法,色谱柱为Lichrospher C_(18)(250 mm×4．6 mm,5μm),以甲醇-水(63:37)为流动相,流速为0．8 mL·min^(-1),检测波长为292 nm。结果:鬼臼毒素在200～800μg·mL^(-1)范围内呈良好的线性关系,r=0．999 9。平均回收率为99．6％,RSD为0．6％;精密度与溶液稳定性RSD分别为0．2％和0．2％。结论:方法快速,重现性、精密度及准确度均能满足鬼臼毒素含量测定要求。Objective： To establish a RP-HPLC method for the determination of podophyllotoxin in microemulsion-based gels. Methods： The RP-HPLC assay was conducted on a Lichrospher C18 column （250 mm × 4.6 mm, 5μm） with a mobile phase composed of methanol：water （63：37 ） with a flow rate of 0.8 mL· min ^- 1 and detection at 292 nm. Results ： The linearity of podophyllotoxin concentration curve was in arange of 200～800μg·mL^-1（r =0.9999）. The average recovery was 99.6% with a RSD of 0.6%. The RSDs of the accuracy and solution stability were 0.2% and 0.2% , respectively. Conclusion ： This rapid, accurate and reproducible RP-HPLC method was useable for quantification of podophyllotoxin in microemulsion-based gels.

关 键 词：鬼臼毒素 微乳凝胶 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]