新型抗癌铂配合物的高效液相色谱分析  

Assay of platinum coordination by high performance liquid chromatography

作　　者：苏强[1] 刘清飞[2] 余尧[3] 王义明[2] 毕开顺[1] 刘伟平[3] 罗国安[2] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]清华大学医学院,北京100084 [3]昆明贵金属研究所,昆明650221

出　　处：《中国新药杂志》2007年第10期791-793,共3页Chinese Journal of New Drugs

摘　　要：目的:建立新型抗癌铂配合物顺式-3,5二异丙基水杨酸根-(1R,2R环己二胺)合铂(Ⅱ)(cis-3,5-dii- sopropylsalylic cyelohexanodiaminoplatinum(Ⅱ),LSPt-DACH)的HPLC分析方法,并应用于其油水分配系数losP的测定。方法:色谱条件:色谱柱Kromasil C_(18)(250 mm×4．6 mm,5μm)反相柱,流动相为甲醇-10 mmol·L^(-1)醋酸铵溶液(70:30),检测波长315 nm,流速1．0 mL·min^(-1),柱温28℃。结果:线性范围0．05～0．60μg,相关系数r=0．999 9;精密度和稳定性良好。LSPt-DACH的油水分配系数10gP值为2．72。结论:所建立的方法简单、灵敏,可作为LSPt- DACH的含量测定方法。Objective ： To establish a HPLC method for the assay of platinum, cis-3, 5-diisopropylsalylic cyclohexanodiaminoplatinum（ II ） （LSPt-DACH） associated with the determination of water-oil partition coefficient （log P）. Methods：The assay was conducted by a HPLC consisted of a Kromasil C18 column （250 mm ×4.6 mm,5μm） with a column temperature at 28°C, an eluate of methanol-10 mmol. L^-1 ammonium acetate solution （70： 30） with a flow rate of 1.0 mL.min^-1 and a detection wavelength at 315nm. Results：The calibrated linear curve of platinum coordination concentration was within 0.05 - 0. 60μg with correlation efficient r = 0. 999 9 and the correlation of y = 7 109.7x ＋ 1 387.8. The log P of this platinum coordination was determined as 2.72. Conclusion： This sensitive and reliable HPLC method was useful for quantification of LSPt-DACH.

关 键 词：铂配合物 高效液相色谱 油水分配系数 

分 类 号：R927.14[医药卫生—药学] R979.1