肝硬化大鼠肝切除术后肠内营养联合白介素-6应用研究  

Experimental observation on the effect of pariteral nutriton and leukin-6 on recovery of hepatic fuanction of liver-removed rat

作　　者：包品红[1] 罗时敏[1] 李旺林[1] 罗建威[1] 

机构地区：[1]广州医学院附属市一人民医院,广东广州510180

出　　处：《中国热带医学》2007年第6期898-900,共3页China Tropical Medicine

基　　金：广州市科技攻关计划项目(2005Z3-E0181);广州市医药卫生科技项目(2006-YB-016)资助

摘　　要：目的探讨肠内营养联合白介素-6在肝硬化大鼠肝切除术后的作用。方法6只正常大鼠作为正常对照组,将24只肝硬化大鼠随机分为术前组6只,肝切除术后1d组6只,肝切除术后行肠内营养(EN)5d组6只,肝切除术后行EN+IL-65d组6只。测定大鼠肝功能、免疫功能、炎症反应和脂质过氧化。结果与EN5d组比较,EN+IL-65d组血清ALB、IGF-1显著升高(P<0.05),外周血CD3+、CD4+、CD4+/CD8+、NK细胞显著升高(P<0.05),外周血CD8+显著下降(P<0.05),血清IFN-γ、TNF-α、MDA显著下降(P<0.05),血清SOD活性显著升高(P<0.05)。结论EN+IL-6可以加快肝硬化大鼠肝切除术后肝功能的恢复,促进肝脏蛋白合成,改善术后免疫功能低下,减轻炎症反应和脂质过氧化损伤。Objective To explore the effect of enteral antriton plus interleukin （IL - 6）on revocery of hepatic function of cirrhotic rats. Methods Six normal mrs were arranged in normal group, 24 rats with liver cirrhosis were randomly divided into four groups each consisted of 6 rats, namely： pre- operation group, post - operation 1 day group, post- operation EN 5 day group and post - operation EN ＋ IL - 6 5 day group. Liver function, immunological function, inflammatory responses, and lipid peroxidation of the rats were determined. Results Compared with the enteral nutrition 5 day group, levels of serum ALB and ICF - 1 were higher （ P 〈 0.05）, the peripheral blood CD3^＋ , CD4^＋ , CD4^＋/CD8^＋ ratio, nature kill cells were higher （ P 〈 0.05）, CD8^＋ was lower （ P 〈 0.05）, serum IFN - γ, TNF - α and MDA content were lower （ P 〈 0.05）, serum SOD was higher （ P 〈 0.05） in the EN ＋ IL - 6 5 day group. Conclusion Posthepateetomy enteral nutrition with IL - 6 in cirrhotic rats can speed up the recovery of liver function, promote liver protein synthesis, ameliorate the damage to immunological function and reduce inflammatory responses as weft as lipid pemxidation damage.

关 键 词：肝硬化 肝切除术 肠内营养 白介素-6 

分 类 号：R575.2[医药卫生—消化系统]