人骨髓间充质干细胞分化为心肌样细胞的研究  

Studies on human mesenchymal stem cells differentiation into cardiomyocytes in vitro

作　　者：李宁 侯相麟[2] 

机构地区：[1]大连血液中心 [2]兰州大学第一医院血液科

出　　处：《中国临床药理学与治疗学》2007年第4期401-404,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究人骨髓间充质干细胞(hMSCs)在体外分离纯化、培养扩增和向心肌样细胞诱导分化的条件。方法:体外分离培养hMSCs,纯化传代至第三、四代,加入不同浓度5-氮杂胞苷(5-Aza)进行不同时间的孵育,用MTT测定细胞的生长活性,确定最佳的浓度和孵育时间。4周后行电镜,免疫组化染色及逆转录-聚合酶链式反应(RT-PCR)检测。结果:诱导后细胞呈心肌样细胞改变,电镜下可见肌丝形成,免疫组化显示部分细胞胞浆α-横纹肌肌动蛋白(α-sarcomeric actin)、肌钙蛋白-T(troponin-T)阳性。RT-PCR检测显示心肌特异转录因子GATA-4、Nkx2.5有表达。结论:hMSCs是骨髓来源的具有多向分化潜能的干细胞,在5-Aza的诱导下可向心肌样细胞分化,可成为心肌损伤移植治疗的理想细胞材料。AIM： To study the mechanisms of the human mesenchymal stem cells differentiating into cardiomyocytes induced by 5-azacyditine. METHODS： The mesenchymal stem cells were separated and purified in vitro, then given 5-azacyditine at three concentrations and at different incubation time. The vitality of the mesenchymal stem cells were assayed with the methylthiazolyl tetrazolium method in order to estimate the best concentration and incubation time. The induced cells were checked by transmission electron microscope, immunohistochemistry and RT-PCR. RESULTS： The induced cell schanged their morphology to that of cardiomyocyte type. Transmission electron microscope revealed striation. Some cells were positive expression of α-sarcomeric actin, troponin-T and cardiac specific transcript factors GATA-4, Nkx2.5. CONCLUSION： The huaman bone marrow-derived mesenchyrnal stem ceils possesses the multiple differentiation potential and can be induced to cardiomyocytes by 5-azacyditine in vitro.

关 键 词：人骨髓间充质干细胞 分化 心肌细胞 体外 

分 类 号：R542.2[医药卫生—心血管疾病]