Construction and Characterization of a cDNA Expression Library for the North American Ginseng Root Tissues  

作　　者：WANG Ying WANG Kun BAO Yong-li WU Yin MENG Xiang-ying LI Yu-xin 

机构地区：[1]Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education [2]Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, P. R. China

出　　处：《Chemical Research in Chinese Universities》2007年第3期310-313,共4页高等学校化学研究（英文版）

基　　金：Supported by the Science Fund for Social Development Program(Modern Chinese Medicine) of Jilin Province(No20011109); Analysis and Testing Foundation of Northeast Normal University

摘　　要：The root of Panax ginseng plant undergoes a specific developmental process to become a biosynthesis and accumulation tissue for ginsenosides. To identify and analyze genes involved in the biosynthesis of ginsenoside, we constructed and characterized a full-length cDNA library for 6-year-old North American ginseng. The titer of primary cDNA library is 1.2 × 10^6 pfu/mL, the titer of amplified library is 2. 6 × 10^10 pfu/mL and the rate of recombinant is above 86%. The insert size ranges from 0. 3 to 2.0 kb. Sequencing results show that 18 of 58 genes are high homologous to the genes （GBRS, GBR3 and GBR1 ） known in GenBank, which are involved in biosynthesis of ginsenoside in North American ginseng plant; 16 of 58 genes are novel genes. The full-length cDNA library of North American ginseng root tissues is essential for the cloning of genes known and it is also an initial key for the screening and cloning of new genes.The root of Panax ginseng plant undergoes a specific developmental process to become a biosynthesis and accumulation tissue for ginsenosides. To identify and analyze genes involved in the biosynthesis of ginsenoside, we constructed and characterized a full-length cDNA library for 6-year-old North American ginseng. The titer of primary cDNA library is 1.2 × 10^6 pfu/mL, the titer of amplified library is 2. 6 × 10^10 pfu/mL and the rate of recombinant is above 86%. The insert size ranges from 0. 3 to 2.0 kb. Sequencing results show that 18 of 58 genes are high homologous to the genes （GBRS, GBR3 and GBR1 ） known in GenBank, which are involved in biosynthesis of ginsenoside in North American ginseng plant; 16 of 58 genes are novel genes. The full-length cDNA library of North American ginseng root tissues is essential for the cloning of genes known and it is also an initial key for the screening and cloning of new genes.

关 键 词：cDNA library North American ginseng SMART 

分 类 号：S567.53[农业科学—中草药栽培]