脂氧素A4对脂多糖活化小鼠巨噬细胞NF-κB和TNF-α转化酶的影响  

Effect of Upoxin A4 on the NF-κB and TNF-α converting enzyme in mice macrophages activated by lipopolysaccharide

作　　者：金胜威[1] 张力[2] 叶笃筠[2] 吴萍[2] 周晓燕[2] 熊维[2] 姚尚龙[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院麻醉科,武汉市430022 [2]华中科技大学同济医学院病理生理学系

出　　处：《中华麻醉学杂志》2007年第4期339-341,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30570726）

摘　　要：目的 研究脂氧素A4（LXA4）对脂多糖（LPS）活化的小鼠巨噬细胞NF-κB及TNF-α转化酶的影响。方法 小鼠RAW 264．7巨噬细胞株培养于细胞培养板,随机分为空白对照组、LPS处理组（LPS 1μg/ml）和LPS＋LXA4组（LPS 1μg/ml＋LXA4 10^-7mol/L）。酶联免疫吸附法测定上清液TNF-α水平,提取细胞总蛋白,Western blot法测定NF-κB p65、IκB-α及TNF-α转化酶含量,荧光素酶报告质粒测定NF-κB活性。结果 LXA4抑制LPS活化的RAW 264．7巨噬细胞IκB-α降解、NF-κB p65核转位及NF-κB活性,同时抑制TNF-α蛋白表达和上调TNF-α转化酶蛋白表达。结论 LXA4通过抑制NF-κB活性和下调TNF-α转化酶表达而减少LPS活化的RAW264．7巨噬细胞分泌TNF-α。Objective To investigate the effect of lipoxin A4 （LXA4） on nuclear translocation of NF-κB and TNF-α converting enzyme activity in RAW 264.7 macrophages activated with lipopolysaccharide （LIPS）. Methods RAW 264.7 macrophages were purchased from Shanghai Cell Biology Research Institute and cultured in RPMI 1640 culture medium in 5% CO2 incubator at 37℃. The macrophages were randomly divided into 3 groups： group A control; group B LIPS and group C LPS ＋ LXA4, LPS 1 μg/ml （final concentration） was added to the macrophages in group B and C, LXA4 10^-7 mol/L （final concentration） was added to the macrophages in addition to LIPS in group C. TNF-α concentration in supernatant was measured by ELISA. The expression of NF-κB p65,IκB-α and TNF-α converting enzyme was detected by Western blotting. Results LXA4 inhibited the degradation of IκB-α, nuclear translocation of NF-κB p65 and NF-κB mediated transcriptional activity in macrophages induced by LIPS. LXA4 also inhibited the TNF-α protein expression and up-regulation of TNF-α converting enzyme protein expression induced by LPS.Conclusion LXA4 can inhibit the release of TNF-α from RAW 264.7 macrophages activated with LIPS through inhibition of NF-κB transcriptional activity and down-regulation of TNF-α converting enzyme.

关 键 词：脂氧素类 脂多糖类 NF-ΚB 金属蛋白酶类 

分 类 号：R686[医药卫生—骨科学]