电针与丁丙诺啡合用对慢性炎性痛大鼠脊髓背角P物质、降钙素基因相关肽的影响  

Effects of electroacupuncture combined with intrathecal buprenorphine on expression of substance P and calcitonin gene-related peptide in spinal dorsal horn in rats with chronic inflammatory pain

作　　者：范隆[1] 王国林[1] 于泳浩[1] 

机构地区：[1]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2007年第4期368-371,共4页Chinese Journal of Anesthesiology

基　　金：天津自然科学基金资助项目（043608911）

摘　　要：目的 评价电针与丁丙诺啡合用对慢性炎性痛大鼠脊髓背角P物质（SP）、降钙素基因相关肽（CGRP）表达的影响,探讨其镇痛机制。方法 鞘内置管成功的成年雌性SD大鼠30只,随机分为5组（n=6）：正常组（A组）、致炎组（B组,大鼠右踝关节腔内注射完全弗氏佐剂50μl）、电针组（C组,大鼠致炎后第4、7、10天电针阳陵泉和足三里,频率2 Hz/100 Hz,强度≤2 mA,30 min/次）、丁丙诺啡组（D组,大鼠致炎后第4天开始,连续7d鞘内注射丁丙诺啡1．5mg）和针药合用组（E组,针药使用方法同C组、D组）。热板法测定大鼠痛敏分数。致炎后第11天取L4-6脊髓,测定SP、CGRP水平。结果 与B组相比,C组、D组、E组痛敏分数降低,患侧脊髓背角SP、CGRP水平减少（P＜0．05）;与C组、D组相比,E组痛敏分数、SP、CGRP水平减少（P＜0．05）。结论 电针和丁丙诺啡能减轻慢性炎性痛大鼠的痛敏反应,针药合用的作用更强,其镇痛机制与减少脊髓背角SP、CGRP表达有关。Objective To investigate the effects of electroacupuncture （EA） combined with intrathecal （IT） buprenorphine on the expression of substance P （SP） and calcitonin gene-related peptide （CGRP） in spinal dorsal horn in rats with chronic inflammatory pain （ CIP）. Methods Thirty adult female SD rats weighing 200-240 g were randomly divided into 5 groups （ n = 6 each） ： A control; B CIP; C EA ＋ CIP; D IT buprenorphine ＋ CIP and E EA ＋ IT buprenorphine ＋ CIP. CIP was induced by injection of complete Freund＇s adjuvant （CFA） 50 td into right tibio-tarsal joint. In group D and E buprenorphine 1.5 μg in 20 μl was injected intrathecally via an IT catheter once a day for 7 consecutive days starting from the 4th day after CIP was induced. In group C and E electrical stimulation of Yardinquan and Zusanli was performed 30 min/day on the 4th, 7th and 10th day after CIP was induced. The paw withdrawal latency to noxious thermal stimulation was measured using hot plate test and compared between the right and left hindpaw. The lumbar segment （L4-6） of spinal cord was removed on the 11th day after induction of CIP for determination of the expression of SP and CGRP in the spinal dorsal horn using immunohistochemistry and computer image analysis system. Results SP and CGRP expression in spinal dorsal horn was significantly increased in CIP group （group B ） as compared with control group and EA and /or IT buprenorphine significantly attenuated the increase in SP and CGRP in spinal dorsal horn induced by CIP. Conclusion Both EA and IT buprenorphine can significantly decrease the hyperalgesia induced by CIP in rats and EA compined with IT buprenorphine provides better effect. Their analgesic effect is closely related to the decrease in SP and CGRP expression in spinal dorsal horn.

关 键 词：电针 丁丙诺啡 疼痛 P物质 降钙素基因相关肽 

分 类 号：R686[医药卫生—骨科学]