羊草光合作用相关基因的克隆与分析  

Cloning and Analysis of Leymus chinensis Photosynthesis Related Genes

作　　者：郝亚琦[1] 刘公社[2] 王丽娟[2] 时珍[2] 王益权[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨陵712100 [2]中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093

出　　处：《西北植物学报》2007年第5期898-902,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国科学院知识创新重大项目(KSCX1-08-03)

摘　　要：在构建了羊草叶片cDNA文库的基础上,利用M13载体通用引物筛选其亚文库,挑选阳性克隆进行测序,将测序结果在NCBI基因库中进行比对,得到一个Rubisco大亚基基因全长序列和Rubisco小亚基基因部分序列,并对其核苷酸及其编码的氨基酸序列进行分析。结果显示,Rubisco大亚基基因长度为1 796 bp,与禾本科大麦、小麦、野雀麦、粗山羊草、旱麦草、异形花草、黑麦等的核苷酸序列同源性达98%以上;羊草的Rubisco小亚基基因部分序列含有一个开放阅读框,其长度为186 bp,编码61个氨基酸,与禾本科的小麦、大麦、燕麦、黑麦以及扁穗雀麦Rubisco小亚基基因氨基酸序列的同源性分别为93%、93%、91%、91%、92%。羊草Rubisco基因的克隆与分析有利于进一步研究其光合作用效率。Based on the leaf cDNA library of Leymus chinensis ,we used the M13 universal primers to screen positive clones to sequence. The sequences were submitted to NCBI for estimating their homogeneity,and we got a Rubisco large subunit gene and a gene fragment of Rubisco small subunit. Rubisco large subunit gene was 1 796 long,which was more than 9B % homologous to the sequences of Rubisco large subunit gene nucleotides in Hordeum capense, Triticum aestivum, Bromus arvensis, Aegilops tauschii, Eremopyrum triticeum, Heteranthelium piliferum et al. ORF of Rubisco small subunit gene was 186 bp long and encoded 61 amino acids. It was 93 %, 93%, 91%, 91% and 92 % homologous to the sequences of amino acid in Hordeum capense, Triticum aestivum, Arena clauda, Secale cereale and Bromus cathartic, respectively. The cloning of Rubisco genes is helpful for the further chinensis. research of photosynthetic efficiency in Leymus chinensis.

关 键 词：Rubisco大亚基 RUBISCO小亚基 羊草 序列分析 

分 类 号：Q789[生物学—分子生物学]