喹胺醇对小鼠血清中细胞因子水平的影响  

Study on the effect of Kuianchun on the level of cytokines in mouse sera

作　　者：乔莉萍[1] 杨志强[1] 李建喜[1] 王学智[1] 梁剑平[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《中兽医医药杂志》2007年第3期9-11,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：中国农业科学院兰州畜牧与兽药研究所基金项目(MYJJ04-03)

摘　　要：目的:观察新兽药喹胺醇对小鼠血清中细胞因子TNF-α、IL-2和IL-6水平的影响。方法:采用放射免疫法检测血清中TNF-α、IL-2和IL-6的含量。结果:中剂量组雄鼠血清中TNF-α含量显著高于对照组和其它药物剂量组(P<0.05),药后7 d雌鼠低剂量组TNF-α含量显著高于对照组和其它药物组(P<0.05);药后7 d雄鼠中剂量组血清中IL-6含量极显著高于对照组(P<0.01),14 d雄鼠中、高剂量组IL-6含量极显著高于对照组(P<0.01),药后7 d雌鼠中、高、最高剂量组IL-6含量显著低于对照组(P<0.05),14 d低剂量组极显著高于对照组(P<0.01);雄鼠血清中IL-2含量在7 d和14 d各组间均无显著差异。结论:喹胺醇对小鼠血清中细胞因子TNF-α、IL-2和IL-6水平的影响可能存在性别差异和剂量差异。Objective： To explore the effects of Kuianchun on the level of TNF-α,IL-2 and IL-6 in sera of mice.Methods： Radioimmunologic techniques were used to detect the levels of TNF-α,IL-2 and IL-6.Results： The content of TNF-α in sera of middle dose group male mice was significantly higher than control group and others（P〈0.05）,low dose group female mice on 7 d higher than control group and others（P〈0.05）;The content of IL-6 in sera of middle dose group male mice was significantly higher than control group on 7 d（P〈0.01）,middle and high dose group higher than control group on 14d （P〈0.01）,middle,high and the highest dose group female mice lower than control group on 7 d（P〈0.05）,low dose group significantly higher than control group on 14 d （P〈0.01）;the sera level of IL-2 was no significant difference between each group male mice on 7d and 14d .Conclusion：The effect of Kuianchun on the levels of TNF-α ,IL-2 and IL-6 in sera of mice may vary with sex and dose.

关 键 词：细胞因子 肿瘤坏死因子 白细胞介素 小鼠 

分 类 号：S859.7[农业科学—临床兽医学]