干扰素基因定向进化库的建立及初步筛选  

Established and Screened of Directed Evolution Library of Interferon Gene in Swine

作　　者：吴明福[1] 蒋忠军[2] 马玉海[2] 唐博恒[2] 王君伟[1] 

机构地区：[1]东北农业大学,哈尔滨150030 [2]广州军区疾病预防控制中心,广州510069

出　　处：《热带医学杂志》2007年第5期426-428,共3页Journal of Tropical Medicine

基　　金：广州市科技攻关项目(No.200223-B4022)

摘　　要：目的构建α干扰素基因家族定向文库。方法利用DNA shuffling技术对人、鸡和猪α干扰素基因进行人工改造,结合噬菌体展示技术构建噬菌体基因改组文库,并对文库进行验证。结果构建了α干扰素基因家族定向文库,其容量为3.5×107pfu;测序得到了3条与猪、人α干扰素基因高度同源的序列。结论得到了1个高质量的α干扰素基因家族定向文库,为今后筛选高效的α干扰素奠定了基础。Objective To construct a library of α-interferon gene. Methods A library of human, swine, and avian interferon gene was constructed using both DNA shuffling technique and phage display technology. Results The titers of un-amplified and the amplified library are 3.5 ×10^7 pfu/ml,respectively. 3 of the human interferon DNA sequences show a high degree of homology with pig interferon. Conclusion A high quality interferon gene library was constructed successfully.

关 键 词：Α干扰素 DNA改组 文库 

分 类 号：R373[医药卫生—病原生物学]