毛头鬼伞多糖抗烟草花叶病毒(TMV)活性研究初报  

A Preliminary Study on Anti-TMV Activity of Polysaccharide from Coprinus comatus

作　　者：吴艳兵[1] 谢荔岩[1] 谢联辉[1] 林奇英[1] 林诗发[1] 

机构地区：[1]福建农林大学植物病毒研究所,生物农药与化学生物学教育部重点实验室,福建福州350002

出　　处：《中国农学通报》2007年第5期338-341,共4页Chinese Agricultural Science Bulletin

基　　金：福建省教育厅专项科学基金项目"一种新型植物源病毒防治剂的研制"(N0.K04037);福建省科技厅重点资助项目"无公害植物源农药的研制"(99-Z-193);福建省科技厅三项费用"福建省植物病毒学重点实验室建设补助费"(K03005)

摘　　要：【目的】为探讨毛头鬼伞多糖(Coprinus comatus)的生物活性,对毛头鬼伞多糖抗烟草花叶病毒(Tobacco mosaic virus,TMV)活性进行测定,明确活性部位,为进一步分离纯化提供理论依据。【方法】从毛头鬼伞子实体和菌丝体提取多糖,并初步纯化,采用半叶法和叶碟法对抗病毒活性进行测定。【结果】毛头鬼伞多糖对TMV具有较强的体外抑制和抗病毒侵染作用,对TMV具有明显的体内抑制复制效果;在TMV接种前施用可以显著降低TMV侵染能力。【结论】毛头鬼伞多糖具有较好的抗TMV活性,为其进一步开发利用提供理论依据。[OBJECTIVE]To explore bio-activity of polysaccharide from Coprinus comatus, antiviral activity of polysaccharide from Coprinus comatus on tobacco mosaic virus （TMV） was investigated. It can provide theoretical evidence for purification. [METHODlPolysaccharide of fruiting body and polysaccharide of mycelium were isolated from Coprinus comatus, and their antiviral activity were researched. [RESULTS] Polysaccharide from Coprinus comatus had strong antiviral activity of preventing infection of virus, affected significantly multiplication of TMV and can reduce infection when applied before TMV inoculation. [CONCLUSION]Polysaccharide from Coprinus comatus had strong antiviral activity.

关 键 词：毛头鬼伞 多糖 抗病毒活性 

分 类 号：S432.41[农业科学—植物病理学]