急性心肌缺血期大鼠丘脑束旁核神经元5-HT_(1A)受体mRNA表达变化的研究  

Study of the expression of 5-HT_(1A) receptor mRNA of the neurons in parafascicular nucleus following acute myocardial ischemia in rats

作　　者：温建忠[1] 郭政[1] 

机构地区：[1]山西医科大学第二医院麻醉科,太原030001

出　　处：《中国急救医学》2007年第6期539-542,共4页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金(No.30371372)

摘　　要：目的观察急性心肌缺血期大鼠丘脑束旁核神经元5-HT1A受体mRNA不同时间点表达的变化,以探讨5-HT1A受体在丘脑束旁核痛觉整合和调制中的作用。方法健康成年雄性SD大鼠24只,随机分为四组:对照组,即非冠状动脉扎闭(C组)组,扎闭冠状动脉(coronary artery occlusion,CAO)1h组(CAO1h组),扎闭冠状动脉3h组(CAO3h组)和扎闭冠状动脉6h组(CAO6h组)。对照组仅在开胸后冠状动脉左前降支下穿线不予结扎;CAO各组则扎闭冠状动脉左前降支。在预定的时点处死动物,取含有大鼠丘脑束旁核的脑片行原位杂交。杂交结果检测采用IDA-2000数码显微图像分析系统进行半定量分析。结果CAO后1、3、6h大鼠丘脑束旁核神经元5-HT1A受体mRNA的表达均较对照组明显增强(P<0.05),随缺血时间延长其表达有逐渐增加的趋势,在CAO后6h表达最强(P<0.05)。结论急性心肌缺血可诱发大鼠丘脑束旁核5-HT1A受体mRNA表达增强,5-HT1A受体参与急性心肌缺血伤害性刺激在丘脑束旁核的调制。Objective The purpose of this study was to investigate the changes in expression of 5 - HTIA receptor mRNA in rat parafascicular nucleus （Pf） induced by coronary artery occlusion （CAO）. Methods Twenty - four adult male SD rats randomly divided into four groups： group C： the control group, with non-CAO; group CAO 1 h： with CAO for 1 hour; group CAO 3 h： with CAO for 3 hours; group CAO 6 h ： with CAO for 6 hours. Coronary artery was occluded except for group C. Rats were killed at scheduled time points, and then the brains were dissected in order to perform in situ hybridization （ ISH ） of brain tissue cryo - section. Brain slices of Pf were examined and analyzed semi - quantitively for expression of 5 - HTIA receptor mRNA by using ISH technique with IDA - 2000 digital micro - image analysis systems. Results The expression of 5 - HTIA receptor mRNA in rat Pf increased significantly at 1 h, 3 h and 6 h after CAO as compared with that in group C （ P 〈 0. 05 ）. The value was the highest at 6 h in group CAO 6 h （ P 〈 0. 05 ）. Conclusion Myocardial ischemia alone could cause the increase in the expression of 5 - HT1A receptor mRNA of the neurons in rat Pf and it could serve as evidence showing that 5 - HTIA receptor in Pf might be involved in central modulation of nociception evoked by acute myocardial ischemia.

关 键 词：心肌缺血 丘脑束旁核 5-HT1A 受体MRNA 大鼠 

分 类 号：R541[医药卫生—心血管疾病]