裙带菜PSⅡ中心复合物的分离和活性鉴定  

Isolation and Activity Identification of PSⅡ Core Complexes From Undaria pinnatifida

作　　者：刘洪艳[1] 王广策[2] 

机构地区：[1]天津科技大学海洋科学与工程学院,天津300450 [2]中国科学院海洋研究所,山东青岛266071

出　　处：《海洋科学进展》2007年第2期233-237,共5页Advances in Marine Science

基　　金：国家自然科学基金项目--藻类光系统反应中心复合物的分离纯化(39890390);天津科技大学科学研究基金资助项目--紫球藻光生物反应器及藻红蛋白分离纯化技术的研究(20050210)

摘　　要：以褐藻裙带菜(Undaria pinnatifida)为实验材料,建立一条能够分离出有光活性PSⅡ复合物的技术路线。采用蔗糖密度梯度超速离心的方法,以去污剂SDS为增溶剂(SDS∶Chl=20∶1,4℃增溶20 min),蔗糖质量分数梯度为10%,15%,20%,30%,40%,50%和60%,从裙带菜中成功地分离出5条含色素的蛋白质复合物带。通过对裙带菜各色素蛋白复合物的光谱分析并进行了DCIP的光还原测定和化学法的氧化还原差示光谱测定,确定了20%和30%层带的复合物为PSⅡ复合物,具有DCIP的光还原活性。利用离子交换层析的方法并通过光谱鉴定分析得到了比较纯化的PSⅡ中心复合物。An isolation and purification technique of light active PS Ⅱ complexes from brown algae Undaria pinnatifida was developed. Five pigment-protein complexes were isolated from the SDS-treated Undaria pinnatifida thylakoid membrane by using sucrose density gradient ultracentrifugation method, and the isolation was made by using solubilizer SDS（SDS ： Ch1=20 ： 1, solubilizing for 20 min at 4℃） and sucrose density gradient of 10%,15%,20%,30%,40%,50% and 60%. It is shown from theDCIPphoto-reduction determination and redox differential spectrophotometric results that the compounds in 20% and 30% sucrose layers were identified as PS Ⅱ complexes with DCIP photo-reduction activity. The purified PS Ⅱ core complexes were obtained by using ion exchange chromatography and identified by spectrometry.

关 键 词：裙带菜 PSⅡ复合物 光谱 

分 类 号：Q949.28[生物学—植物学]