银杏叶片三种酶活性变化与抗疫霉菌关系的研究  

Study on relationship between resistance against Phytophthora micotianae and variation of three enzymes activities of Ginkgo biloba

作　　者：林敏敏[1] 廖咏梅[1] 周志权 

机构地区：[1]广西大学农学院,南宁530005 [2]广西产品质量监督检验院,南宁530022

出　　处：《广西植物》2007年第3期513-517,526,共6页Guihaia

基　　金：广西自然科学基金(桂科自9811016)~~

摘　　要：将疫霉菌离体接种于银杏叶片,发现不同龄期银杏叶片对疫霉菌的抗病性有差异,叶龄为100d的抗病性较强,叶龄为20d的抗病性较弱。不同银杏品种叶片对疫霉菌的抗性也有差异,潮田1号和大佛手2号较抗病,大佛手1号和桂G86-1较感病。不同龄期叶片接种后,其过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的酶活性变化与抗病性成正相关,而多酚氧化酶(PPOD)的酶活性变化与抗病性没有相关性;不同银杏品种叶片接种后,品种间的抗病性与PPOD酶活性变化成正相关,而POD和PAL的酶活性变化与品种间的抗病性没有相关性。可见,POD、PPOD和PAL的酶活性变化没有明显规律性,不能作为衡量银杏叶片抗病性的生化指标。The results of Phytophthora nicotianae inoculated on Gindgo biloba leaves showed that leaves with different age had different disease resistance,in which it had stronger disease resistance at 100d age while 20d age leaves had weaker disease resistance;among different varieties,Chaotian No. 1 and Dafoshou No. 2 showed stronger disease resistance while Dafoshou No. 1 and GuiG86-1 had weaker disease resistance. Results of three enzymes activities detection showed that peroxidase （POD） and phenylalanine ammonia-lyase （PAL） enzymes activities had positive relationship with disease resistance while polyphenoloxidase （PPOD） hadn＇ t. Among different varieties, PPOD enzyme activity had positive relationship with disease resistance while POD and PAL hadn＇ t. The detection results suggested that POD,PPOD and PAL enzymes activities did not have regular vatiation in leaves of different age and different varieties,so it＇s not advisable to choose the three enzymes activities as the criterion of disease resistance.

关 键 词：银杏 疫霉菌 抗病性 酶活性 

分 类 号：Q945[生物学—植物学]