尖蜜拉的快繁技术  

Rapid Propagation Techniques for Artocarpus Champeden Spreng.

作　　者：张翠玲[1] 文慧婷[1] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533

出　　处：《热带作物学报》2007年第1期51-53,共3页Chinese Journal of Tropical Crops

摘　　要：以尖蜜拉老茎干上的不定芽作外植体,采用从不定芽→丛生芽→完整植株的繁殖途径进行繁殖。结果表明尖蜜拉不定芽外植体在温度(26±2)℃,光照强度1500～2000lx、光照时间12h/d条件下,茎萌发最适培养基为MS+6-BA2.0mg/L+NAA0.1mg/L+Vc0.5g/L;茎增殖最适培养基为MS附加6-BA3.0mg/L+NAA0.5mg/L;茎生根最适培养基为1/2MS+IBA0.5mg/L+NAA3.0mg/L+AC0.5g/L;生根时温度可适当上升为(28±2)℃,光照强度2000lx,光照时间12h/d。Adventitious buds on the trunk ofArtocarpus champeden （Loureiro） Spreng. were used as explants for rapid propagation. The result showed that the best medium for shoot growth is MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L ＋Vc 0.5 g/L under the light 12 h/day with 1 500-2 000 lx at （26±2） ℃. The best medium for rapid propagation of shoots is MS＋6-BA 3.0 mg/L＋NAA 0.5 mg/L. Rooting medium is 1/2 MS＋NAA 3.0 mg/L＋IBA 0.5 mg/L. At the rooting of the shoots, the temperature was raised to （28±2） ℃ and the light strength to 2 000 lx with the light period being 12 h/day.

关 键 词：尖蜜拉 不定芽 组织培养 

分 类 号：S667.9[农业科学—果树学] S343.6[农业科学—园艺学]