多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的动态观察  

Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《重庆医科大学学报》2007年第7期676-678,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金(30671835;30500423和30200239);教育部重点项目(205131);重庆市教委科研基金(KJ050313);重庆市科委科研基金(03-43-8);重庆市卫生局科研基金(03-2-099)。

摘　　要：目的:动态观察多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的变化。方法:将疫苗采用鼻腔内接种免疫BALB/C鼠,在免疫后0、2、4、6、8、10、12、14、16和18周各剖杀4只小鼠取脾,分离脾细胞,流式细胞仪检测脾CD_4^+和CD_8^+T淋巴细胞亚群的百分比,同时设有PBS对照。结果:疫苗接种组的脾CD_4^+和CD_8^+T细胞亚群分别在免疫后2～12周和2～18周升高,分别在免疫后6和10周达最高水平。结论:CD_4^+T细胞亚群数目在多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫早期(2～6周)显著增加,Objective.To dynamically observe changes of subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Eml4-3-3 vaccine of Echinococcus multilocularis（Em）.Methocls：BALB/C mice were intranasally vaccinated with the vaccine and killed to get spleen at 0,2,4,6,8,10,12,14,16 and 18w of immunization respectively. Splenocytes were separated to measure subsets of CD4^＋ and CD8CF cells by FACsort, PBS served as control. Results：In the groups of immunization, CD4^＋ and CD8subsets increased obviously at 2-12w and 2-18w respectively, which reached the highest level at 6 and lOw respectively. Conclusion：The number of CD4^＋ subsets of splenocytes increases remarkably in the early stage （2-6week）by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Eml4-3-3 vaccine.

关 键 词：多房棘球绦虫 重组BCG-EmⅡ/3疫苗 重组BCG-EM14-3-3疫苗 脾细胞亚群 

分 类 号：R383.3[医药卫生—医学寄生虫学]