肥胖基因表达产物与肥胖的关系  

RELATIONSHIP BETWEEN OBESE GENE EXPRESSIVE PRODUCT AND OBESITY

作　　者：高连如[1,2] 赵云涛[1,2] 朱智明[1,2] 栾佐[1,2] 石湘芸[1,2] 熊鉴然[1,2] 杨晔[1,2] 田青[1,2] 张肇康[1,2] 唐朝枢[1,2] 

机构地区：[1]海军总医院 [2]北京医科大学心血管基础研究所

出　　处：《中华内科杂志》1997年第3期180-182,共3页Chinese Journal of Internal Medicine

摘　　要：为了解肥胖蛋白（ＯＰ）５７－９２活性肽段［（５７－９２）］与肥胖的关系，应用肥胖蛋白及其特异性兔抗人ＯＰ（５７－９２）血清建立了液相竞争放射免疫分析法，观察了正常人和小鼠ＯＰ组织分布特征及肥胖患者ＯＰ含量变化。并通过外源性给予１２５Ｉ－ＯＰ活性肽段［（１１６－１６７）和（９３－１０５）］观察了其体内分布。结果显示：小鼠脑组织含有丰富的ＯＰ，明显高于脂肪组织，肝及肌肉组织中未检出ＯＰ，脂肪组织中雌性小鼠ＯＰ含量明显低于雄性。正常人血浆ＯＰ含量为１９４．３±１７．７ｎｇ／Ｌ，小鼠血浆ＯＰ为２２５７．８±１７１．９ｎｇ／Ｌ。外源性给予１２５Ｉ－ＯＰ肽段后体内分布以肾脏最丰富，肝肺次之，血浆半衰期４、５分钟。肥胖成人及肥胖小儿血浆ＯＰ含量均较正常对照组明显减低，且ＯＰ水平与体重指数，血糖、血胆固醇、甘油三脂呈显著负相关。提示：ＯＰ含量变化与肥胖发病学有重要关系。In order to study the relationship between obese protein and obesity. A radioimmunoassay for human plasma obese protein (OP) was established using human OP (57 92) and its specific antiserum. We observed the distribution and the characteristics. Of OP in normal human and mouse tissue and changes of plasma OP in obese patients. We also observed the tissue distribution after intravenous injection of 125 I labeled OP fragments (116 167) and (93 105) in rats. The results were as follows. The contents of OP were much higher in brain tissue than those in abdominal adipose tissue; levels of OP in liver and in skeletal muscle tissue were zero; the contents of abdominal adipose tissue were higher in female mice than those in male mice; the contents of plasma OP in normal human beings were 194.3±17.7 ng/L, while those in mice were 2257.8±171.9 ng/L. It was also found that the administered OP fragments were widely distributed in various tissue and organs including the brain. Kidney was the richest in the OP fragments; liver and lung ranked second. The half time of the OP fragments in plasma clearance was about 4,5 min. The contents of OP in obese adults and in obese children were much lower than those in normal control group. There was significant negative correlation between OP levels and body mass index, serum concentration of glucose, cholesterol and triglycerides. So the present study indicates the important relationship between the changes of OP contents and the pathogenesis of obesity.

关 键 词：肥胖病 基因表达 

分 类 号：R589.2[医药卫生—内分泌]