凋亡相关基因Bcl-2和bax及iNOS在流感病毒感染后小鼠嗅上皮的表达  

Apoptosis related genes——Bcl-2,bax and iNOS,expressed in the olfactory epithelium of mice infected with influenza virus

作　　者：陈志宏[1] 倪道凤[1] 高扬[2] 林嘉友[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉-头颈外科 [2]中国医学科学院中国协和医科大学基础医学研究所免疫系

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第11期510-512,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：科技部项目;科技基础性工作和社会公益研究专项面上项目(No:2003DIBJ077)

摘　　要：目的:研究凋亡相关基因Bcl-2、bax和诱导型一氧化氮合酶(iNOS)在流感病毒感染后小鼠嗅上皮的表达,探讨其对嗅感觉神经元凋亡的调节作用。方法:采用半定量RT-PCR法检测流感病毒感染后小鼠嗅上皮Bcl-2、bax和iNOS的mRNA表达水平。结果:流感病毒感染后:①嗅上皮表达Bcl-2mRNA相对恒定;②嗅上皮表达baxmRNA早期显著增加,恢复期逐渐回落;③嗅上皮表达iNOSmRNA在一段较短的时期内显著增加,1周后逐渐下降至不可测水平。结论:凋亡相关基因Bcl-2、bax和iNOS可能在流感病毒感染所致的嗅感觉神经元凋亡中起重要的调节作用。Objective：To study the expression of apoptosis related genes,Bcl-2, bax, and iNOS in the olfactory epithelium of mice infected with influenza virus, and to discuss how they regulate apoptosis of the olfactory sensory neurons. Method：The expression levels of apoptosis related genes were detected with semi-quantity RT-PCR. Result：①The expression levels of Bcl-2 mRNA remain relatively constant after virus inoculation; ②The expression levels of bax mRNA increased massively, and decreased gradually;③The expression levels of iNOS mRNA increased significantly in a short period, and then fell down to the undetectable level gradually. Conclusion：The apoptosis related genes,Bcl-2, bax, and iNOS may play important roles in regulation of apoptosis of olfactory sensory neurons of mice infected with influenza virus.

关 键 词：流感病毒 嗅感觉神经元 基因 Bcl-2 基因 bax 一氧化氮合酶 

分 类 号：R349.64[医药卫生—基础医学]