人类巨细胞病毒感染对脐血红系祖细胞同源盒B6基因表达的影响  

Influence of human cytomegalovirus infection on the expression of HOXB6 gene in human cord blood erythroid progenitor cell

作　　者：冉伶[1] 刘文君[1] 陈艾[1] 胡晓[1] 陈红英[1] 

机构地区：[1]泸州医学院附属医院儿科,四川泸州646000

出　　处：《中国实用儿科杂志》2007年第6期452-454,共3页Chinese Journal of Practical Pediatrics

基　　金：四川省教育厅重点科研项目(2004A058)

摘　　要：目的研究脐血红系祖细胞的HOXB6基因的表达情况以及人类巨细胞病毒(HCMV)感染对红系祖细胞的HOXB6基因表达的影响,以探讨HCMV导致脐血红系祖细胞损伤的机制。方法在泸州医学院附属医院儿科以实时荧光定量PCR技术(FQ-RT-PCR)检测人类巨细胞病毒感染和(或)用全反式维甲酸(ATRA)处理后HOXB6基因的表达水平。结果人脐血红系祖细胞可表达HOXB6基因;HCMV感染后,红系祖细胞HOXB6基因的表达明显下调;ATRA能显著上调红系祖细胞HOXB6基因的表达水平,且能够在一定时间内上调HCMV感染后的红系祖细胞HOXB6基因的表达。结论HCMV感染能诱导脐血红系祖细胞HOXB6的表达下调,HC-MV有可能通过调控HOXB6基因表达异常而引起红系祖细胞的增殖障碍。Objective To investigate the expression of HOXB6 gene in human cord blood colony forming unit erythroid （CFU-E） , and the influence of human cytomegalovirus （ HCMV ） infection on the expression of the gene in order to explore the possible mechanism of HCMV-induced malformations. Methods The expression of HOXB6 gene in erythroid progenitor cells infected with HCMV and/or treated with ATRA were determined by fluorogenic quantitative reserve transcription polymerase chain reaction （FQ-RT-PCR） method. Results Erythroid progenitor continually expressed HOXB6 gene. The expression of HOXB6 gene was reduced after HCMV infection. Treated with all-trans retinic acid （ ATRA ） , the expression of HOXB6 gene in erythroid progenitor cells was increased. The expression of HOXB6 gene infected with HCMV and treates with ATRA was reduced in given time. Conclusion HCMV infection significantly down-regulated the expression of HOXB6 gene. The result suggests that the abnormal expression of HOXB6 gene induced by HCMV may play an important role in HCMV-indueed abnormal hematogenie damage.

关 键 词：人类巨细胞病毒 HOXB6基因 红系祖细胞 全反式维甲酸 

分 类 号：R72[医药卫生—儿科]