建立沾化冬枣离体再生体系的防褐化研究  

Study on in Vitro Regeneration System of Winter Ziziphus jujube var-Zhanhua

作　　者：张孝霖[1] 刘雪红[1] 段代祥[1] 刘俊华[1] 吴涛[1] 游晶晶[1] 

机构地区：[1]滨州学院生命科学系,山东滨州256603

出　　处：《安徽农业科学》2007年第15期4444-4445,共2页Journal of Anhui Agricultural Sciences

基　　金：滨州学院自然科学基金项目(BZXYQNLG2005015)

摘　　要：系统研究了外植体类型,植物激素,光温环境因子,抗氧化剂(PVP)和吸附剂(VC)对建立沾化冬枣离体再生苗褐化的影响。结果表明:水培芽和低浓度的细胞分裂素可减轻离体再生苗褐化,培养初期,低温、暗处理、缩短转接培养基周期2、.0～5.0 g/L的PVP和一定浓度的VC可有效抑制植株体褐化。The explants type,plant hormone,environmental factor of light and temperature,antioxidants（PVP） and the absorbent（vitamin C） which results in the browning of Winter Ziziphus jujube var-Zhanhua were studied.The results showed that the bud under water-culture and low concentration of cytokine can reduce browning.In early stage,the low temperature, dark, short culturing cycle,2.0 - 5.0 g/L PVP and certain concentration of Vc can effectively in hibit browning.

关 键 词：沾化冬枣 离体再生 褐化 外植体 

分 类 号：Q943.1[生物学—植物学]