磺胺间二甲氧嘧啶人工抗原的合成与鉴定  

Synthesis and Identification of the Sulfadimethoxine College Artificial Antigen

作　　者：鲁晓翠[1] 朱金凤[2] 张改平[1] 邓瑞广[1] 侯玉泽[3] 王自良[4] 彩鸿翔[3] 王青[5] 

机构地区：[1]河南省动物免疫学重点实验室,河南郑州450002 [2]河南农业职业学院,河南中牟451450 [3]河南科技大学食品与生物工程学院,河南洛阳471003 [4]河南科技学院动物科学系,河南新乡453003 [5]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《安徽农业科学》2007年第15期4544-4545,4587,共3页Journal of Anhui Agricultural Sciences

基　　金："十五"国家重大科技专项(2001BA804A30-08)

摘　　要：用重氮化方法将SDM偶联于载体蛋白BSA和OVA上,合成免疫抗原BSA-SDM和包被抗原OVA-SDM,通过紫外扫描(UV)、SDS-PAGE凝胶电泳试验。结果显示:BSA-SDM和OVA-SDM的UV与BSA、SDM的相比均发生了改变,出现了明显的特征峰。这表明半抗原和载体偶联成功。用BSA-SDM免疫BALB/C小鼠,间接ELISA测定多抗上清(pAb)效价。结果表明,获得了高效价的pAb,为SDM残留免疫学检测方法的建立奠定了基础。Diazotization was used to conjugate SDM to carrier protein BSA and OVA and obtained immunizing antigen BSA-SDM and coating antigen OVA-SDM.UV and SDS-PAGE were used to identify SDM artificial antigen.The results showed that UV spectrogram of BSA-SDM and OVA-SDM were changed compared with BSA and SDM,the characteristic peak of BSA-SDM was shown obviously.The high-titer anti-SDM pAb was produced in sera of immunized BALB/C mice,which made it possible to establish immunoassay of SDM residues in feed and animal food.

关 键 词：磺胺间二甲氧嘧啶 人工抗原 合成 鉴定 

分 类 号：S859.79[农业科学—临床兽医学]