雪旺细胞在大鼠脱细胞异体神经基膜管中迁移的研究  

A STUDY ON MIGRATION OF SCHWANN CELLS IN ACELLULAR NERVE ALLOGRAFT OF RAT

作　　者：王碧菠[1] 戴传昌[1] 殷德明[1] 金羽青[1] 祁佐良[1] 董佳生[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海200011

出　　处：《中国修复重建外科杂志》2007年第6期582-585,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的研究大鼠自体雪旺细胞在脱细胞异体神经基膜管中的迁移情况。方法取SD大鼠坐骨神经20mm,用化学萃取法制备脱细胞神经,行大体观察、HE、抗层黏蛋白（Anti-laminin）染色。另取32只雌性SD大鼠,体重250～300g,切取自体坐骨神经2mm,置于分别长10mm的两段大鼠异体脱细胞坐骨神经基膜管之间,形成22mm长的基膜管-自体神经嵌合体,与同样长度的单纯脱细胞神经基膜管埋入肌间隙中,于5、10、15和20d取材,行HE、S-100免疫组织化学染色。结果制备的脱细胞神经基膜管外观呈半透明;HE染色示基膜管内未见细胞核存在,基膜管部分不连续;Anti-laminin染色示基膜管深褐色。基膜管-自体神经嵌合体于术后各时间点HE染色均可见细胞存在,第15天开始可见S-100阳性雪旺细胞;单纯神经基膜管在各时间点HE染色中均可见细胞存在,未见S-100阳性雪旺细胞。结论大鼠坐骨神经的自体雪旺细胞可迁移至两侧异体脱细胞神经基膜管远端,为嵌合自体神经的异体脱细胞神经基膜管修复长段周围神经缺损提供了理论依据。Objective To study the migration of Schwann cells from the nerve autograft in the acellular nerve allograft of the rats in vivo. Mehtods The sciatic nerves （20 mm long） of the SD rats were harvested and prepared for the acellular nerve grafts by the chemical extraction. Then, they were observed by the gross view, HE staining, and Anti-laminin staining, respectively. Another 32 female SD rats weighing 250-300 g were obtained for the study. A 2-mm-long nerve autograft was interposed between the two 10-mm-long nerve allografts to form a 22-mm-long composite. Then, the composite was placed in the muscle space, together with a sole 22-mm-long nerve allograft as a control. They were harvested at 5,10,15 and 20 days, respectively, and were then given the HE staining and the S-100 staining. Results The acellular nerve graft was semitransparent under the gross view. HE staining showed that no cell was observed within the nerve graft. Anti-laminin staining showed that the basal membrane was partially interrupted, with a positive result （dark brown）. All the nerve grafts in both the groups exhibited the existence of the cells. The S-100 positive cells were observed from the 15th day at the far ends of the two allografts of the composite; however, there were no such cells observed within the sole nerve allograft. Conclusion Schwann cells from the sciatic nerves （2 mm long） of the rats can migrate in the acellular nerve allograft to the far ends of the neighboring 10-mm-long nerve allografts at 15 days after operation, which offers the theoretical basis for the repair of the long-range nerve defect by the composite of the acellular nerve allografts with the interposed nerve autograft.

关 键 词：雪旺细胞 体内培养 脱细胞神经基膜管 迁移 S-100免疫组织化学染色 

分 类 号：R651.3[医药卫生—外科学]