小鼠胚胎神经干细胞在猫全脑缺血再灌注损伤模型中的存活、迁移及分化  

Survival, migration and differentiation of mouse embryonic neural stem cells in global brain ischemical reperfusion injury model

作　　者：林绿标[1] 章翔[1] 林旭妍[2] 李侠[1] 宋蕾[1] 梁景文[1] 

机构地区：[1]第四军医大学西京脑科医院神经外科,陕西西安710032 [2]汕头市第二人民医院眼科,广东汕头515041

出　　处：《中华神经外科疾病研究杂志》2007年第3期207-209,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30370512)

摘　　要：目的观察小鼠神经干细胞在猫全脑缺血再灌注损伤模型中的存活、迁移及分化。方法参照四血管阻塞法建立猫全脑缺血再灌注损伤模型,将原代培养的小鼠神经干细胞移植入模型动物脑中。一个月后,以免疫荧光技术检测移植干细胞的存活、迁移及分化情况。结果缺血再灌注损伤模型制作成功7例,成功率70%;免疫荧光法检测小鼠神经干细胞移植到猫前脑后一个月仍有大量存活,部分细胞分化为神经元或神经胶质细胞,并向脑组织中迁移。结论小鼠神经干细胞在猫全脑缺血再灌注损伤模型中能够存活、迁移并分化。Objective To observe surviving, migrating and differentiating of mice neural stem cells in global brain ischemical reperfusion injury models of cat. Methods According to four vessels occlusion methods, global ischemical reperfusion injury models were established in 10 cats, while 2 cats in sham operated group as control. Primary culture mice neural stem cells were transplanted into global brain ischemia/refusion models, but cats in sham operated group were injected the same volume of phosphate buffered solution （PBS） as control. After a menth, these cells were detected by immunofluorescence technique. Results Seven global brain ischemical reperfusion injury models were established successfully and the achievement ratio was 70%. Two cats in sham operated group were all survival. Many mice neural stem cells were detected by immunofluorescence in forebrain of cats after transplantation for a month, some of the cells were differentiated into neuron and glial cells, and some of the cells were migrated into brain tissue of cats. Condttsion Mice neural stem cells can survive, migrate and differentiate in global brain ischemical reperfusion injury models of cats.

关 键 词：神经干细胞 全脑缺血模型 移植 免疫荧光法 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]