睾酮对损伤的血管组织血管细胞黏附分子-1的表达和内膜增殖的影响  

Effects of testosterone on VCAM-1 expression and neointimal proliferation of injuried vascular tissue

作　　者：赵子牛[1] 付海霞[2] 许家琍[3] 蒋锡嘉[3] 李庚山[3] 

机构地区：[1]河南省人民医院心血管外科,河南郑州450003 [2]河南省人民医院心内科,河南郑州450003 [3]武汉大学人民医院心内科,湖北武汉430060

出　　处：《中国现代医学杂志》2007年第10期1200-1203,共4页China Journal of Modern Medicine

摘　　要：目的观察内外源性睾酮对球囊损伤的血管组织血管细胞黏附分子-1(VCAM-1)的表达和内膜增殖的影响。方法30只雄性家兔随机等分为替代组、单纯去势组和对照组,前两组动物行去势术(切除双侧睾丸和附睾),后一组动物行相应假手术;均于去势术后2周行右侧髂动脉内皮剥脱术,替代组同时给予十一酸睾酮。脱内皮术后2周将动物处死,取损伤血管段,应用免疫组织化学法观察血管组织VCAM-1的表达、应用HE染色观察血管病理形态的变化。结果与单纯去势组和对照组相比,替代组脱内皮术后2周损伤血管组织VCAM-1表达明显增强[(0.3465±0.0084)vs(0.3109±0.0056)及(0.3180±0.0099),分别P<0.001和0.01],损伤血管内膜面积和内膜/中膜面积比值明显增大[(1.65±0.39)mm2vs(0.65±0.18)mm2及(0.75±0.21)mm2,(0.92±0.20)vs(0.38±0.07)及(0.43±0.09),均P<0.01],而前两组之间上述指标相比差异无显著性(均P>0.05)。结论雄性兔去势后补充该实验剂量的外源性睾酮可促进损伤的血管组织表达VCAM-1和促进内膜增生,而内源性睾酮水平短时降低对损伤血管组织VCAM-1的表达和内膜增生无影响。[Objective] To study the influences of endogenous and exogenous androgen on the expression of vascular cell adhesion molecule-1 （VCAM-1） and neointimal proliferation of injuried vascular tissue. [Methods] Thirty male rabbits were randomly divided into three groups： replacement group, only castrated group and control group. The two former groups were castrated and the control group were sham operated. All animals were performed deendothehalizing balloon injury in fight iliac artery. Replacement group was received testosterone undecanonate replacement therapy. After 2 weaks of endothelial denudation, all animals were killed, VCAM-1 expression were tested with immunohistoehemistry and morphological changes were observed with hematoxylin and eosin （HE） staining. [Results] Compared with only castrated group and control group, VCAM-1 expression, the neointimal area and the ratio of intimal/medial area were increased in replacement group （P 〈0.001 or P 〈0.01）.There were no siginificanfly differences between only castrated group and control group（P 〉0.05）. [Conclusion] When male rabbits were castrated, exogenous testosterone replacement treatment could promote the expression of VCAM-1 and neointimal formation of injuried vascular tissue, while the declining endogenous testosterone didn＇t effect them in a short tenn.

关 键 词：雄激素 血管细胞粘附分子-1 血管成形术 兔 

分 类 号：R466.11[医药卫生—临床医学]