细脚拟青霉多糖对乙醇诱导L-02细胞损伤的保护作用研究  

Study of the Hepatoprotective Effect of Paecilomyces tenuipes Polysaccharides on Alcohol-Induced L-02 Cell Injury

作　　者：胡云良[1,2] 李宝剑[3] 金丽琴[4] 

机构地区：[1]温州医学院检验医学院 [2]温州医学院附属二院检验科,浙江温州325027 [3]温州医学院药学院 [4]温州医学院基础学院生化教研室

出　　处：《中国药学杂志》2007年第11期824-828,共5页Chinese Pharmaceutical Journal

基　　金：浙江省中医药科研基金资助课题(2004C121);温州市科技计划项目资助课题(Y2004A008)

摘　　要：目的探讨细脚拟青霉多糖(PtPs)对乙醇诱导肝细胞损伤的体外保护作用。方法采用RT-PCR分别检测乙醇诱导损伤的乙醇组、PtPs处理组(PtPs+乙醇)及正常对照组L-02细胞肿瘤坏死因子-α(TNF-α)mRNA和热休克蛋白90(HSP90)mRNA的表达;检测细胞培养上清液中黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)活性及丙二醛(MDA)含量;检测细胞匀浆中三磷酸腺苷酶(ATPase)活性,并对各组细胞进行Annexin-FITC标记染色观察凋亡细胞情况。结果与乙醇组比较,乙醇+PtPs组细胞内TNF-α mRNA表达下降(P<0.01),HSP90 mRNA表达增加(P<0.05或P<0.01);细胞培养上清液中SOD活性升高、XOD及LDH活性下降、MDA含量下降(P<0.05或P<0.01);细胞匀浆中ATPase活性上升(P<0.05或P<0.01);PtPs处理后可见乙醇诱导的L-02细胞凋亡和坏死被明显抑制。结论PtPs对乙醇诱导肝细胞的损伤具有保护作用。OBJECTIVE To investigate the protective effect of Paecilomyces tenuipes polysaccharides （PtPs）on alcohol-induced hepatocyte injury in vitro. METHODS RT-PCR was used to detect the expressions of tumor necrosis factor-or （TNF-α） mRNA and heat shock protein 90 （HSP90）mRNA in L-02 cells of alcohol group, PtPs disposed groups （PtPs ＋ alcohol）and normal control group. The levels of xanthinoxidase （ XOD）, superoxide dismutase （ SOD）, lactic dehydrogenase （ LDH）, malondialdehyde （ MDA ） in supernatant and adenosine triphosphatase（ATPase） in cell homogenate were determined. The cells in each group were stained by Annexin-FITC and the apoptosis was observed with fluorescent microscope. RESULTS Compared with those in the alcohol group, the expression of TNF-α mRNA was deereased（P 〈0. 01 ） and that of HSP90 mRNA was inereased（P 〈0. 01 or P 〈0. 05） in PtPs disposed groups. The activity of SOD increased and the levels of XOD, LDH, MDA decreased in supematant of PtPs disposed groups（ P 〈 0. 01 or P 〈 0. 05 ）. The activity of ATPase in cell homogenate increased （P 〈 0. 01 or P 〈 0. 05 ）. PtPs significantly inhibited apoptosis and death of L-02 cells induced by alcohol. CONCLUSION PtPs at a certain concentration could protect hepatocyte from the injury induced by alcohol.

关 键 词：细脚拟青霉 多糖 L-02细胞 乙醇 细胞凋亡 肝损伤 

分 类 号：R965[医药卫生—药理学]