牛精原干细胞研究进展  

The Progress of Bovine Spermatogonial Stem Cells Researches

作　　者：吴建英[1] 胡铁军[2] 岳占碰[1] 张学明[1] 李子义[1] 李德雪[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]吉林省长春皓月清真肉业股份有限公司,长春130062

出　　处：《中国农学通报》2007年第6期28-31,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然基金"小鼠精原干细胞转染人t-PA基因的研究"(30200195);"精原干细胞自增殖调控的分子机制"(30471246);吉林大学博士启动基金"牛精原干细胞的分离富集及体外培养"(2005001)资助

摘　　要：精原干细胞(spermatogonial stem cells,SSCs)是雄性生殖系干细胞,位于睾丸曲细精管基膜上,既具有自我更新潜能,又具有定向分化潜能,是自然状态下出生后动物体内在整个生命过程中进行自我更新并能将基因传递至子代的惟一成体干细胞。根据国内外最新相关进展,系统评述了牛SSCs的生物学特性、分离纯化、冷冻保存、体外培养、永生细胞系的建立及移植等方面的现状及问题。Spermatogonial stem cells （SSCs）, the postnatal male germline stem cells, reside on the base membrane of the seminiferous tubules in testes. They are not only capable of self-renewing and differentiating but also the only adult stem cells in normal postnatal body that undergo self-renewal throughout life and transmit gene to offspring. According to the related reports most recently, the biological properties separation, purification, cryopreservation, in vitro culture, establishment of immortalized cell line and transplantation of bovine spermatogonial stem cells were discussed.

关 键 词：精原干细胞 生物学特性 分离培养 移植 牛 

分 类 号：S858.23[农业科学—临床兽医学]