山羊GH基因5′侧翼序列多态及生物信息学分析  

Polymorphisms and Bioinformatics Analysis of the Goat Growth Hormone Gene in the 5′flanking Sequence

作　　者：李中原[1] 王锋[1] 庞训胜[1] 王瑞芳[1] 王子玉[1] 

机构地区：[1]南京农业大学动物科技学院,南京210095

出　　处：《中国农学通报》2007年第6期42-46,共5页Chinese Agricultural Science Bulletin

基　　金：农业部农业结构调整重大技术研究专项"良种肉羊快速繁育关键技术研究与示范"(05-07-01B);农业部优势农产品重大技术示范推广项目"肉羊人工授精与舍饲快速育肥技术示范与推广"(2004-24)

摘　　要：试验采用PCR-SSCP方法,分析了黄淮山羊、长江三角洲白山羊以及波尔山羊168只个体GH基因5′侧翼区两个位点的遗传多态性,同时分析了三个群体是否为平衡群体。结果如下:在GH基因的两个位点上均发现了多态性,其中第1对引物出现6种基因型,存在2处突变;第2对引物出现3种基因型,存在3处突变。群体分析表明,第1个位点三个群体都处于非平衡状态,说明该位点有经过人工选择的可能;而第2个位点三个群体则都处于平衡状态。进一步利用生物信息学软件分析GH基因的5′侧翼序列,发现在其多态位点处存在IA-1、PIT-1、Gsh-2、C/EBP等多个分值较高的转录因子结合位点,其中264位T→C的突变导致PIT-1和Gsh-2结合位点的消失,至于这些位点是否会影响到GH基因的表达,进而影响到山羊个体的生长发育还需作进一步的研究。The polymorphisms of two sites of GH gene in the 5＇ flanking region from 168 Huanghuai goats,Changjiangsanjiaozhou white goats and Boer goats were analyzed by PCR-SSCP, At the same time, the three communities were analyzed to check whether they were in balance. Results： both of the two sites of GH gene werb polymorphic, there were six genotypes and two mutations with the first pair primers, three genotypes and three mutations with the second pair primers, communities analysis shows that all the three breeds in the first site were in imbalance, which suggests that artificial selection might be possible in this site; and all the three breeds in the second site were in balance. Furthermore, GH gene in the 5＇ flanking sequence were analyzed by bioinformatics software assay, which shows that transcription factor combining sites with high score, such as IA-1,PIT-1,Gsh-2,C/EBP et al, exist in the polymorphic sites, and the mutation of T→C （264） results in the disappearance of PIT-1 and Gsh-2 combining sites, Further studies need to be carried out to check such sites will affect the expression of GH gene, and the growth performance of goat.

关 键 词：PCR-SSCP 山羊 GH基因 多态性 生物信息学 

分 类 号：S827[农业科学—畜牧学]