猪Musclin基因片段克隆与序列分析  

Molecular Cloning and Sequence Analysis of Porcine Musclin

作　　者：王伟杰[1] 郭豫杰[1] 李卫华[1] 林茂旺[1] 赵蕾[1] 杨国宇[1] 

机构地区：[1]河南农业大学动物生理生化实验室,郑州450002

出　　处：《中国农学通报》2007年第6期72-74,共3页Chinese Agricultural Science Bulletin

基　　金：河南省重点科技攻关项目(编号:0523010500)

摘　　要：【研究目的】克隆并分析猪musclin基因;【方法】肌肉组织提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD19-T连接后转化E.coliDH5α,检测阳性克隆并测序;【结果】克隆的猪musclin基因片段与人、大鼠、小鼠同源性分别为86%、78%、75%,预测的氨基酸序列含有“KKKR”结构和与小鼠ANP、BNP、CNP蛋白的同源性区域;【结论】克隆了猪musclin基因片段并注册GenBank(Ac-cession.EF369511)。[Objective] To clone and analysis the porcine musclin. [Method]Total RNA was extracted from muscle tissue and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19Tvector, and then transformed into competent cells of E. coli DH5ct.The sequence was analyzed to identify the recombinant plasmid. [Results]Identity analysis showed that the musclin nucleotide sequence shared 86%, 78% and 75% homology with that of human , rat and mouse. The predicted peptide contained a ＂KKKR＂ motif and the region homologous to mouse ANP, BNP, and CNP. [Conclusion]The porcine partial musclin gene was successfully cloned in present study and the sequence has been submitted to GenBank（Accession.EF369511）.

关 键 词：猪 musclin 克隆 序列分析 

分 类 号：S811.3[农业科学—畜牧学] Q785[农业科学—畜牧兽医]