基质金属蛋白酶-9、组织金属蛋白酶抑制剂-1在喉癌中的表达及其临床意义  

EXPRESSION OF MMP-9 AND TIMP-1 IN LARYNGEAL CARCINOMA AND ITS CORRELATION WITH CLINICAL SIGNIFICANCE

作　　者：李为[1] 白文中[2] 田凤艳[3] 

机构地区：[1]河北省唐山市妇幼保健院耳鼻喉科,063000 [2]北京市海军总医院耳鼻喉科 [3]河北省唐山市妇幼保健院中医科,063000

出　　处：《中国煤炭工业医学杂志》2007年第7期757-759,共3页Chinese Journal of Coal Industry Medicine

摘　　要：目的探讨基质金属蛋白酶(MMP-9)及组织金属蛋白酶抑制剂(TIMP-1)二种蛋白在喉癌中的表达及其临床意义。方法应用流式细胞术检测50例原发喉癌患者新鲜手术切除癌组织及50例相应癌旁组织(距肿瘤边缘0.5cm)与16例非喉部疾病患者的正常喉粘膜组织中MMP-7、MMP-9及TIMP-1的表达。结果MMP-9在喉癌组织中的表达量(FI=1.24±0.15)及阳性表达率(100%)明显高于癌旁组织(FI=1.05±0.12)(72%)和正常粘膜组织(FI=1.0±0.02)(0%),比较差异均有统计学意义(P<0.01)。TIMP-1在喉癌组织中的表达量(FI=0.85±0.16)及阳性表达率(56%)明显低于癌旁组织中的表达量[FI=0.97±0.18,(84%)]和正常喉粘膜组织[FI=1.0±0.13,(88%)],比较差异均有统计学意义(P<0.01);MMP-9及TIMP-1在癌旁组织和正常喉粘膜组织间的表达差异无统计学意义(P>0.05)。结论在喉癌组织中,MMP-9的高表达和TIMP1低表达在喉癌的发生、发展、转移过程中有重要作用,二者联合检测对喉癌的预后评估可能更有价值,并且为临床治疗提供理论指导。Objective To investigate the expression of MMP- 9 and TIMP- 1 in Laryngeal Carcinoma and its Clinical Significance. Methods The expression of MMP - 9 and TIMP - 1 were detected using FCM in tumor tissues of 50 patients with laryngeal carcinoma and meanwhile 50 para - carcinoma tissues and 16 normal laryngeal mucosa samples were also studied as controls. Results The quantitative （FI = 1.24 ± 0.15） and qualitative （100 %） expression of MMP- 9 in laryngeal carcinoma tissues was remarkably higher than those in parecarcinoma（FI = 1.05 ± 0.12）, （61.10%） and in normal laryngeal mucosa tissues （FI = 1.0 ± 0.02）（0 % ）. The quantitative （FI = 0.85 ± 0. 16） and qualitative （100 % ） expression of TIMP- 1 in laryngesl carcinoma tissues was obviously lower than those in para - carcinoma （FI = 0.97± 0.18） （560%） and normal laryngeal mucosa （FI = 1.0 ± 0.13） （88%） tissues. There wasn＇t distinct statistic significance in both MMP-9 and TIMP-1 between the expression of para - carcinoma and normal laryngeal mucosa tissues（P〉0.05）. Conclusion The higher expression of MMP- 9 and the lower expression of TIMP - 1 in Laryngeal Carcinoma is important for progression and metastasis of laryngeal carcinoma. If they are detected simultaneously, it may be more helpful to predict the prognosis of patients with Laryngeal carcinoma, and offer theoretic information for choice of clinical treatment.

关 键 词：喉癌 基质金属蛋白酶-9 组织金属蛋白酶抑制剂-1 流式细胞术 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]