HPLC法测定柴芍止痛颗粒中芍药苷的含量  

Determination of Paeoniflorin in Chaishaozhitong Granule by HPLC

作　　者：刘崇俊[1] 吴宏丽[1] 

机构地区：[1]大连市中医医院,辽宁大连116013

出　　处：《中华中医药学刊》2007年第6期1280-1281,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：建立HPLC法测定柴芍止痛颗粒中芍药苷含量的方法。方法：高效液相色谱法。Hypersil ODS2（4.6mm×250mm,5μm）,流动相：乙腈-0.1%磷酸溶液（15∶85）,流速：1mL/min,检测波长：230nm。结果：芍药苷在0.2-1.6μg间呈良好线性关系,r=0.9998,回收率98.60%,RSD为1.34%。结论：该方法重复性好、简便易行,可作为柴芍止痛颗粒中芍药苷含量的质控方法。Objective： To establish the quality, standard of Chaishaozhitong Granule. Methods： Using HPLC with Hyperil ODS2 （4.6mm×250mm ,5 μm）, The mobile phase was acetonitrile - 0.1% phosphoric acid solution（ 15： 85 ） with 1 mL/min of flow late, and the detection wavelength was at 230nm. Results ： Paeoniflorin showed good linear relationship at the range of 0.2 -1.6μg,The recovery was 98.60% and RSD was 1.34%. Conclusion： The methed was highly repeatable and accurate.

关 键 词：柴芍止痛颗粒 芍药苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]