Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment  

作　　者：SUN Qing-quan ZHANG Ying RONG Ting-zhao DONG Shu-ting ZUO Zhen-peng 

机构地区：[1]Shandong Agricultural University/State Key Laboratory of Crop Biology, Taian 271018, P.R.China [2]Sichuan Agricultural University, Yaan 625014, P.R.China

出　　处：《Agricultural Sciences in China》2007年第6期652-656,共5页中国农业科学（英文版）

摘　　要：In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.

关 键 词：MAIZE inbred line Barstar gene particle bombardment transgenic plant molecular detection 

分 类 号：S513[农业科学—作物学]