不同浓度钙离子对凝血Ⅰ、Ⅱ及Ⅲ因子的作用  

Effects of Different Concentrations of Calcium Ion on the Blood clotting Factors Fibrinogen(Ⅰ),Prothrombin(Ⅱ) and Fibrin Stabilizing Factor( ⅩⅢ )

作　　者：李佩国[1] 张继东[1] 李蕴玉[1] 李双安[1] 王洪发[1] 

机构地区：[1]河北农业技术师范学院

出　　处：《中国兽医学报》1997年第1期75-77,共3页Chinese Journal of Veterinary Science

基　　金：河北省教委基金

摘　　要：家兔血液与不同浓度ＣａＣｌ２溶液混合后，随着Ｃａ２＋浓度的增加血凝时间延长，当血中Ｃａ２＋浓度达０．０５４ｍｏｌ／Ｌ时，出现抗凝；０．７２ｍｏｌ／ＬＣａＣｌ２溶液抗凝兔血置３７℃水浴３０，１２０ｍｉｎ后，再以生理盐水稀释，血液仍能恢复凝固。表明ＣａＣｌ２抗凝血中的纤溶系统未被激活，其抗凝作用具有可逆性；其中的凝血Ⅲ因子无变性、降解、失活现象，对纤维蛋白原含量无影响。Effects of different concentrations of calcium chloride (CaCl 2) on the clotting time of rabbit blood were studied and the reversibility of coagulation activity of factor ⅩⅢ , content of fibrinogen and activity of thrombin were determined. The experimental results showed that the clotting time prolonged with the concentratiom of Ca 2+ to be getting higher and it was uncoagulated when the Ca 2+ concentration was up to 0.54 mol/L. The blood which anticoagulated by 0.72 mol/L of Ca 2+ in water was reversely clotted when it was dipped in water both at 37℃ for 30 min or 120 min and diluted with physiological saline. The results indicated that the blood fibrinolysis system was not activated and the clotting function of CaCl 2 was reversible. There were no denaturation, degradation and deactivation of blood stabilizing factor ⅩⅢ and no effect on fibrinogen content, but it could abviously inhibit the activity of thrombin (4 000 U/L).

关 键 词：钙离子 家兔 血液凝固 凝血ⅩⅢ因子 纤维蛋白质 

分 类 号：S852.2[农业科学—基础兽医学]