许旺细胞与PLGA共同移植于大鼠全横断脊髓损伤的实验研究  

Research about schwann cells and PLGA implanted to rat transected spinal cord

作　　者：万虹[1] 李德志[1] 杨飞[2] 历俊华[1] 王身国[2] 王忠诚[1] 

机构地区：[1]首都医科大学北京市神经外科研究所,100050 [2]中国科学院化学研究所

出　　处：《中华外科杂志》2007年第12期843-846,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金国际合作研究(30540450581);973课题(2005CB522704)

摘　　要：目的观察许旺细胞与 PLGA 组织工程材料共同移植于大鼠全横断脊髓损伤处对脊髓修复的影响。方法许旺细胞与 PLGA 体外共同培养后行扫描电镜观察,将其共移植于大鼠横断性脊髓损伤处,不同时间行 BrdU/MBP 免疫组化、天青-美蓝染色、透射电镜、运动功能评分(BBB)、运动诱发电位(MEP)、股四头肌复合肌肉动作电位(CMAP)、体感诱发电位(SEP)检测。结果扫描电镜下许旺细胞在 PLGA 内生长良好。6个月后移植物内可见 BrdU/MBP 免疫组化双染阳性细胞,天青-美蓝染色和透射电镜观察可见新生的髓鞘及形成髓鞘的许旺细胞。BBB 评分各组间无显著性差异,MEP 和 SEP 检测移植后6个月所有大鼠波形仍未恢复,但 T_(11-12)椎间隙可记录到 SEP 波。结论许旺细胞与 PLGA 具有良好的组织相容性,共同移植于损伤脊髓可促进轴突生长及髓鞘再生,但没有与损伤远端联系。Objective To investigate the recovery of rat transected spinal cord injury after implantation of Schwann cells combined with poly （lactide-co-glycolide） （PLGA）. Methods Schwann cells were expanded, co-cultured with PLGA for 9 days in vitro, and then analyzed with scanning electron microscope （SEM）. Rat spinal cord at the level of T9 was transected. Schwann cells labeled with BrdU and PLGA scaffold were implanted to injury site. After 1, 3, 6 months, BrdU/MBP immunohistochemistry double staining, semi-thin sections stained thionin and ultra-thin section were performed to investigate myelin renew. BBB open field locomotion, motor evoked potential （MEP）, compound muscle action potential （CMAP） and somatosensory evoked potential （SEP） were recorded. Results Schwann cells grew well on PLGA under SEM. BrdU/MBP double positive cells would been seen, remyelination was thin and formed by Schwann cells at 6 months later under electron microscope （EM）. BBB behavioral tests revealed no significant difference in recovery comparing with experiment group and control group. The results of MEP, CMAP and SEP showed no significant improvement in the conduction of spinal cord. Conclusions There are the compatibility between Schwann cells and PLGA. Although remyelination was found in morphology, function conduction of spinal cord failed to be established.

关 键 词：许旺氏细胞 脊髓损伤 髓鞘再生 诱发电位 

分 类 号：R651.2[医药卫生—外科学]