人精液液化的显微镜下特征观察  

Characteristics of human semen by microscopic observation during liquefaction

作　　者：岳焕勋[1] 蒋敏[1] 李福平[1] 林丽[1] 张思孝[2] 

机构地区：[1]四川大学华西第二医院男科,成都610041 [2]四川大学华西医院泌尿科

出　　处：《中华检验医学杂志》2007年第6期652-654,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的比较观察精浆内凝胶状物质消失前后精液分析主要指标的差异，了解该现象在判定精液液化中的意义。方法体外采精后立即充池入精子计数板，置光学显微镜下观察其液化过程中凝胶状物质的形态变化，并于胶状物质消失前后进行精液分析。结果人精液液化过程中可以通过显微镜观察到精浆中凝胶状物质呈现特征性的形态变化，32份精液分析结果显示，前向运动精子和精子活动率在此形态变化前后分别为（30．3±12．4）％和（45．0±14．9）％、（44．9±12．7）％和（59．0±15．3）％，差异有统计学意义（t=-1．130和5．023，P均〈0．01），而精子密度在液化前后分别为（55．3±33．9）×10^6／ml和（62．1±32．3）×10^6／ml，差异无统计学意义（t=1．180，P〉0．05）。结论通过显微镜观察到精液中凝胶状物质呈特征性变化，该特征可作为一种客观和简易可行的判定精液液化的手段。Objective To compaire the physical characteristics of semen morphology before and after semen coagulation-liquefaction under microscope and understand the significance of these characteristics for the determination of semen liquefaction. Methods Semens were placed in sperm counting chamber immediately after collection. The morphological changes of the seminal gelatinous substance during liquefaction were observed under microscope and analyzed. Results Morphological changes during semen liquefaction can be clearly observed. Among 32 samples of semen, forward sperm progression and motility were （30.3 ±12.4）% and （45.0 ±14.9）%, （44.9 ±12.7）% and （59.0 ±15.3）%, before and after the liquefaction,respectively. The differences were statistically significant （t = - 1. 130,5. 023 ,P 〈0. 01 ）, while the variation of the semen density was （55.3 ± 33. 9 ） × 10^6/ml vs. （ 62. 1 ± 32. 3 ） × 10^6/ml which was not significant statistically （ t = 1. 180 ,P 〉 0.05 ）. Conclusions Microscopic observation of the seminal gelatinous substance is a simple and feasible method for objective measurement of sperm liquefaction. These findings suggest it may provide pathological significance on semen liquefaction abnormality in infertility clinic.

关 键 词：精液 显微镜检查 精液不液化症 

分 类 号：R446.1[医药卫生—诊断学]