代用蛋壳培养体系对鸡胚骨组织中碱性磷酸酶活性的影响  

THE EFFECT OF CULTURING CHICKEN EMBRYOS IN RECIPIENT EGG SHELLS ON ACTIVITY OF BONE ALP

作　　者：李赞东[1] 樱井富士郎 李海昌 黄劲松 李相芝 张曼夫[1] 齐顺章[1] 

机构地区：[1]北京农业大学生物学院

出　　处：《畜牧兽医学报》1997年第1期44-48,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：以双黄鸡蛋的蛋壳为代用蛋壳，将正常孵化７２ｈ的鸡胚转移至其中进行孵化，取孵化至１０、１２、１４、１６、１８和２０ｄ的鸡胚，测定其骨中碱性磷酸酶（ＡＬＰ）的活性。同时分析正常孵化鸡胚骨中ＡＬＰ的活性做为对照。结果表明：（１）对照组和实验组的ＡＬＰ活性均随着胚胎日龄的增加而升高，尤其是１６ｄ后上升得很快；（２）１４日龄以前的鸡胚，实验组与对照组相比，ＡＬＰ活性无明显差异；（３）孵化后期同日龄鸡胚相比，实验组ＡＬＰ活性高于对照组的ＡＬＰ活性，差异显著。上述结果表明，在代用蛋壳中培养的鸡胚。Recipient shells were prepared from double—yolked eggs We cut off the blunt end of the recipient egg shells and made a hole of 40mm in diameter Then chicken egg contents were discarded The fertilized eggs were normally incubated for 72h We transferred the chicken embryos into the recipient egg shells Chicken embryos in recipient egg shells were incubated until 10,12,14,16,18,20d,respectively We collected the leg bones(osfemoris) of embryos of different development stage and tested the activity of ALP(alkaline pholphatase)of bones From this experiment we can draw the following conclusions:(1)The activity of ALP of experiment group and control group tended to increase with the development of embryos After 16 days of incubation,it increased sharply;(2)During the late period of hatching the activity of ALP in experiment group was significantly higher than that in control group;(3)The activity of ALP of chicken embryos before 14 days between experiment group and the control significant difference was not found These conclusions indicated that the lack of calcium in embryos,incubated in recipient shells,leaded to bone metabolic abnormal

关 键 词：ALP 代用蛋壳 鸡胚 腿骨钙 骨组织 碱性磷酸酶 

分 类 号：S852.165[农业科学—基础兽医学]