结缔组织生长因子在胃硬癌形成机制中的作用  

Function of connective tissue growth factor in formation of scirrhous gastric carcinoma

作　　者：肖在鹏[1] 胡祥[1] 杨佩满[2] 邵淑娟[2] 胡军[2] 赵瑾瑶[2] 

机构地区：[1]大连医科大学附属第一医院普外科 ,116011 [2]大连医科大学组织学胚胎学教研室

出　　处：《中国医师进修杂志（外科版）》2007年第6期8-11,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的研究结缔组织生长因子（CTGF）表达与胃癌大体分型的关系，并探讨其在胃硬癌间质纤维化形成中的作用。方法采用免疫组化染色SP方法，检测28例胃硬癌（胃硬癌组）、30例胃非硬癌（非硬癌组）及30例癌旁良性胃组织（对照组）中CTGF及α-平滑肌肌动蛋白（α-SMA）的表达，应用苦味酸-天狼猩红染色方法检测Ⅰ型与Ⅲ型胶原纤维在以上三组中的表达。结果胃硬癌组中CTGF、Ⅰ型与Ⅲ型胶原纤维的表达水平均明显高于非硬癌组及对照组（P〈0．01）；与对照组相比，非硬癌组中CTGF、Ⅰ型与Ⅲ型胶原纤维的表达显著增强（P〈0．01）；CTGF的表达与胃壁组织的纤维化程度呈正相关（P〈0．01）；α-SMA在胃硬癌组与非硬癌组中的表达差异无统计学意义（P〉0．05）；α-SMA表达与CTGF、Ⅰ型及Ⅲ型胶原纤维的含量无明显相关性（P〉0．05）。结论CTGF在以胶原纤维性间质增生为特点的胃硬癌形成过程中起促进作用，成肌纤维细胞不是胃硬癌纤维化间质的主要来源细胞。Objective To investigate the expression of connective tissue growth factor （CTGF） in different type of gastric carcinoma based on the cancer - stromal relationship and its effect on the formation of scirrhous gastric carcinoma. Methods Immunohistochemical SP method was used to detect the expression of CTGF and α- SMA in 28 eases of seirrhous gastric carcinoma （SC group）, 30 eases of non - seirrhous gastric carcinoma （ NSC group） and 30 eases of para - cancer tissue samples （ control group）. Sirius red staining was performed to assess the expression of type Ⅰ and Ⅲ collagen in the above 3 groups. Results The levels of the expression of CTGF, type Ⅰand Ⅲ collagen were significantly higher in SC group than those in NSC and control group （ P 〈 0.01 ）. In comparison with control group, the expressions of CTGF, type Ⅰ and Ⅲ collagen in NSC group were remarkably increased （P 〈 0.01 ）. The expression of CTGF had a positive relationship with that of type Ⅰ and Ⅲ collagen （P 〈 0.01 ）. The expression of α- SMA had no evident difference between SC and NSC group （P 〉 0.05 ）. There was no significant correlation between the expression of α- SMA and that of CTGF, type Ⅰ and Ⅲ collagen （P 〉 0.05 ）. Conclusions CTGF plays an important role in the formation of scirrhous gastric carcinoma characterized by stromal fibrosis. Myofibroblasts are not responsible for the desmoplastic reaction of scirrhous gastric carcinoma.

关 键 词：结缔组织生长因子 胃硬癌 间质纤维化 Α-平滑肌肌动蛋白 

分 类 号：R692[医药卫生—泌尿科学]