槲皮素分子烙印聚合物的单体优化、性能评价及其在薄层色谱分离中的应用  

Monomers Optimization and Properties Evaluation of Quercetin-imprinted Polymer and Its Application to Thin Layer Chromatography Stationary Phase

作　　者：何建峰[1] 邓芹英[2] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《中药材》2007年第5期588-591,共4页Journal of Chinese Medicinal Materials

基　　金：国家教育部博士点基金(20020558026);广州中医药大学创新基金(K0050035)

摘　　要：以黄酮类化合物槲皮素为模板,选用甲基丙烯酸(MAA)、丙烯酰胺(AM)、N,N-二乙基胺乙基甲基丙烯酸酯(DEAEM)、2-乙烯吡啶(2-VP)为功能单体,制备对槲皮素具有特定亲和性和选择性的分子烙印聚合物,采用平衡结合实验考察它们对底物的吸附特性,发现以离子作用结合的功能单体2-VP和DEAEM对槲皮素具有较好的烙印效果。研究并将这种烙印聚合物作为薄层色谱的固定相,成功地分离了模板分子及其结构类似物。此外本文还考察了聚合物的粒度和重复使用次数对吸附效果的影响。The quercetin-molecularly imprinted polymers with specific affinity and selectivity were prepared by using methacrylic acid （MAA）, acrylamide （AM）, N, N-（diethylamino） ethyl methacrylate and 2-vinylpyridine as functional monomers, respectively. The adsorption properties for template were studied by equilibrium binding experiments. The results showed 2-VP and DEAEM based on ionic interaction with quercetin possessed better imprinting effects. Using the quercetin-imprinted polymers as thin layer chromatographic stationary could successfully separate the template from the other structurally related compounds. In addition, the influence on adsorption effect about the particle size and repeating times of MIP were investigated.

关 键 词：槲皮素 分子烙印聚合物 结合实验 薄层色谱固定相 

分 类 号：R286.02[医药卫生—中药学]