高效液相色谱法测定玄丹巴布剂中丹酚酸B含量  

Quantitative Determination of Salvianolic Acid B in Xuandan Cataplasm by HPLC

作　　者：孟舒[1,3] 邢青峰[1,3] 全艳晖 李淼 张振秋[3] 

机构地区：[1]辽宁省计划生育科学研究院,辽宁沈阳110032 [2]沈阳市福利院,辽宁沈阳110031 [3]辽宁中医药大学,辽宁沈阳110032

出　　处：《解放军药学学报》2007年第3期217-218,共2页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：辽宁省科技厅科技攻关项目;No.2004225003

摘　　要：目的为保证药物安全有效,完善玄丹巴布剂质量标准,对玄丹巴布剂中丹酚酸B进行了含量测定方法的研究。方法HPLC色谱条件:十八烷基硅烷键合硅胶色谱柱kromasilC18(250mm×4.6mm,5μm);甲醇-乙腈-甲酸-水(28∶10∶1∶61)为流动相,检测波长为286nm,流速1.0ml/min。结果丹酚酸B在浓度0.179～0.896μg范围内线性关系良好,回归方程为Y=836807.5X-30356.9。样品的平均回收率为100.5%,RSD为1.42%。结论本方法简便可靠,结果稳定,重复性好,可准确测定玄丹巴布剂中丹酚酸B的含量。Aim To perfect the quality standard of Xuandan cataplasm. Salvianolic acid B was chosen as the quality control index. Methods HPLC conditions ： C18 column （ Kromasil C18 5μm, 250mm×4.6mm）, methol -aceto- nitrile-formic acid-water（28 ： 10：1 ： 61 ） as the mobile phase, detection wavelength ： 286nm, flow rate ： 1.0mL/min. Results Salvianolic acid B has a good linearity within the range of 0. 179 -0. 896μg. The average recovery rate was 100.5% （ RSD = 1.42% ）. Conclusion The procedure is simple and reliable. The results are stable and reproducible. The established method could determine salvianolic acid B from Xuandan cataplasm accurately.

关 键 词：玄丹巴布剂 HPLC 丹酚酸B 

分 类 号：R927.2[医药卫生—药学]