复方氯霉素醇溶液含量测定方法的改进  

Determination of Salicylic Acid and Chloramphenicol in Compound Chloramphenicol Alcoholic Solution by RP-HPLC

作　　者：刘晋华[1] 李玉萍[1] 尤光甫[1] 

机构地区：[1]解放军第251医院,河北张家口075000

出　　处：《解放军药学学报》2007年第3期228-230,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立复方氯霉素醇溶液中水杨酸和氯霉素的含量测定方法。方法采用反相高效液相色谱法,色谱柱:C18(250mm×4.6mm,5μm);流动相:甲醇-水-冰醋酸(50∶50∶0.1);流速:0.8ml/min;检测波长:295nm;柱温:30℃。结果水杨酸在0.2535～2.5350μg.ml-1范围内呈良好线性关系,r=0.9999,平均回收率99.75%,RSD为1.3%;氯霉素在0.2550～2.5500μg.ml-1范围内呈良好线性关系,r=0.9999,平均回收率99.18%,RSD为1.3%。结论方法简便、准确,重现性好。Aim To set up an assay method for determination of salicylic acid and chloramphenicol in Compound Chloramphenicol Alcoholic Solution. Methods The content of salicylic acid and chlorampheni -col was determined by RP-HPLC on a C18 columu （250mm×4.6mm,5μm） . The mobile phase consisted of CH3OH-H2O-HAc. （05：05：0.1 ） . The flow rate was 0.8ml/min, at the detection wavelength of 295 nm and column temperature of 30℃. Results Salicylic acid showed a good linear relationship within a range of 0. 253 5～2. 535 0μg·ml^-1, r =0. 999 9 ; the average recovery was 99.75%, and RSD was 1.3%. The linear range of chloramphenicol was 0. 255 0 - 2. 550 01μg·ml^-11, r = 0. 999 9, and the mean recovery was 99.18 %, with an RSD of 1.3 %. Conclusion This method is simple, accurate and reproducibil.

关 键 词：复方氯霉素醇溶液 HPLC 水杨酸 氯霉素 

分 类 号：R927.2[医药卫生—药学]