垂丝海棠组培再生体系建立的研究  

Establishment of Regeneration System of Malus halliana koehne by Tissue Culture

作　　者：张庆田[1] 夏阳[2] 孙仲序[1] 刘燕苓[1] 陈兴彬[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东省林业科学研究院,山东济南250014

出　　处：《生物技术》2007年第3期73-75,共3页Biotechnology

摘　　要：目的:通过对垂丝海棠(Malus halliana koehne)进行组织培养研究为木本观赏海棠提供技术支持。方法:采用常规组培手段及正交设计试验对外植体消毒时间、快繁培养、叶片愈伤诱导、再生、生根等进行研究。结果:外植体最佳消毒时间:75%酒精30s+0.1%升汞10min;最适增殖培养基为:MS+6-BA0.7mg/L+NAA0.3mg/L,增殖系数可达7;正交设计结果显示激素对叶片愈伤组织诱导影响的因素大小为:2,4-D>6-BA>NAA;再生最佳配方为6-BA6.0mg/L+NAA0.5mg/L再生效率达91.8%;最适生根培养基为:1/2MS+IBA0.3mg/L+蔗糖20g/L。结论:对垂丝海棠组培再生研究取得初步成功。Objective： Technical support would be provided to woody ornamental by tissue culture of Malus halliana koehne. Methods： Conventional tissue culture methods and orthogonal design had been researched for explants disinfection, rapid reproduction, leaves callus induction, regeneration, rooting. Results： The optimum sterilization times of explants were 75% alcohol 30s ＋ 0.1% mercuric chloride 10min .The most suitable medium for the initiation of adventitious buds was MS medium supplemented with 0.7mg/L 6 - BA and 0.3mg/L NAA, and the ratio of buds propagation is 7 ; The results showed harmone on the leaves orthogonal design factors affecting the size of the callus induction： 2 4 - D 〉 6 - BA 〉 NAA;The best regeneration tissue culture was MS＋ 6 - BA 6.0 rag/L＋ NAA 0.5mg/L; The regeneration efficieney was 91.8%. The best rontage euhure medium is 1/2MS＋ IBA 0.3mg/L＋ sucrose 20g/L was most suitable for the footage. Conclusion： Tissue culture of Malus halliana koehne was success.

关 键 词：垂丝海棠 组织培养 再生 观赏植物 

分 类 号：S722.37[农业科学—林木遗传育种]