喉癌细胞中S100A8新的相互作用蛋白质的鉴定  

Novel partners of S100A8 identified in laryngeal cancer cell lines

作　　者：富伟能[1] 郭艳[1] 黄带发[1] 尚超[1] 孙开来[1] 

机构地区：[1]中国医科大学医学遗传学教研室,沈阳110001

出　　处：《中华医学遗传学杂志》2007年第3期266-270,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30171008）,国家863项目（2002BA711A08-18）

摘　　要：目的探索S100A8相互作用蛋白在喉癌发生、发展中的可能机制。方法应用抗S100A8抗体通过免疫沉淀的方法从喉癌细胞系Hep-2中分离与S100A8相互作用的蛋白质。用基质辅助激光解析电离飞行时间质谱仪分析目的蛋白条带。根据这些目的蛋白条带的肽指纹谱,用Mascot软件预测其相应的蛋白质。用P-Match软件预测这些蛋白质的NF-kappa B结合位点。用免疫共沉淀方法证实其中一个蛋白质与S100A8相互结合的能力。结果获得了4种与S100A8相互作用的新的蛋白质,它们分别是假想蛋白质LOC80154（hypothetical protein LOC80154）、MHCclass Ⅰ HLA-B、T-box1异构体C相似蛋白质（similar to T-box1 isoformC）和肌纤维膜相关蛋白1（sarcolemmal associated protein 1）。这4种蛋白质均具有NF-kappa B的结合位点,其中MHCclass Ⅰ HLA-B是NF-kappa B通路中的一个成员,我们首次证实该蛋白质具有结合S100A8的能力。结论本研究获得的S100A8新的伴侣可能是NF-kappa B通路的成员。MHCclass Ⅰ HLA-B与S100A8的结合提示S100A8可能作为新成员与包括HLA-B在内的其他蛋白质在NF-kappa B通路中发挥作用。这些发现为进一步研究S100A8在喉癌发生中的分子机制提供了新线索。Objective To explore mechanism of S100A8 in the oncogenesis and development of laryngeal cancer. Methods Proteins interacting with S100A8 were isolated from laryngeal cancer cell lines Hep-2 by immunoprecipitation assay with anti-S100A8 antibody. The target bands were cut out and identified by maxtrix assisted laser desorption/ ionization time of flight （MALDI-TOF）. The peptide mass fingerprinting data of the proteins identified were analyzed based on the Mascot database. The NF-kappa B binding sites of the proteins were predicted by P-Match software. The binding ability of one of the proteins to S100A8 was confirmed by co-immunoprecipitation and inanunocytochemistry metheds. Results Four proteins interacting with S100A8 were obtained, which were hypothetical protein LOC80154, MHC class Ⅰ HLA-B, similar to T-box 1 isoform C and sarcolemmal associated protein 1. The four genes were predicted to have NF-kappa B binding sites. MHC class Ⅰ HLA-B, which is one of targets in NF-kappa B pathway, was first confirmed to have the binding abihty to S100A8. Conclusion The novel partners of S100A8 identified in the study might be involved in NF-kappa B pathway. The binding ability of MHC class Ⅰ HLA-B to S100A8 imphes that S100A8 might function as a new member with other proteins including HLA-B in NF-kappa B pathway. These findings provide a new clue to further study on the molecular mechanism of S100A8 in the genesis of laryngeal carcinomas.

关 键 词：喉癌 S100A8相互作用蛋白 NF-KAPPA B通路 人类白细胞抗原B 

分 类 号：R686[医药卫生—骨科学]